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Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST1811
Products putative malate dehydrogenase
Plasmid ID 0107
Vector  
Purification Lot ST1811
Purified Date 2004.4.5-
Protein conc. (mg/ml) 11.9
Total volume (ml) 1.2
Total protein (mg) 14.3

Culture Lot:

date host cell scale label weight of bacteira (g)
        22.5

Cell Lysis and Heat Treatment:

 
 
 
 
 
 
 
 
 

Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Iso 6ml 20mM Tris-HCl(pH8.0),5mM B-ME,0.60M AS 0.60M AS 0M AS 3 0.60M AS
2 Resource Q 6 ml 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 2.0M NaCl 3 0.116M NaCl
3 Bio-Scale CHT10I 10mM KPi(pH7.0),5mM B-ME 10mM KPi 500mM KPi 2 10mM KPi
4 Superdex200HR10/30 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl     4  
5 Resource S 6 ml 20mM MES(pH6.0),5mM B-ME 0M NaCl 2.0M NaCl 4 0.34M NaCl
6 HiPrep 26/10 Desalting 53ml 20mM Tris-HCl(pH8.0),5mM B-ME        
7            
8            
9            

Comment:

 

2011.10.20

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