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Available from Riken BioResource Center.

Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST1790
Products conserved hypothetical protein
Plasmid ID R0468
Vector  
Purification Lot ST1790_2
Purified Date 2004.9.14-
Protein conc. (mg/ml)  
Total volume (ml)  
Total protein (mg)  

Culture Lot:

date host cell scale label weight of bacteira (g)
         

Cell Lysis and Heat Treatment:

 
 
 
 
 
 
 
 
 

Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Phe 6ml 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS 1.5M AS 0M AS   between 1.4M AS and 1.0M AS
2 Resource Q 6 ml 20mM Tris-HCl(pH8.0),5mM B-ME       0.1M NaCl
3 Bio-Scale CHT10I 10mM KPi(pH7.0),5mM B-ME       150mM KPi
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Comment:

 

2011.10.20

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