Species | |
---|---|
Locus tag | ST1790 |
Products | |
Plasmid ID | R0468 |
Vector | |
Purification Lot | ST1790_1 |
Purified Date | 2003.7.29 |
Protein conc. (mg/ml) | 1.5 |
Total volume (ml) | 0.5 |
Total protein (mg) | 0.75 |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
20.1 |
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | ToyoScreen PPG 650M | 20mM Tris-HCl(pH8.0),5mM B-ME,1.2M AS | 1.2M AS | 0M AS | 5 | Flow through |
2 | ToyoScreen PPG 650M | 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS | 1.5M AS | 0M AS | 4 | 1.2M AS |
3 | Resource Q 6 ml | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 1M NaCl | 4 | Flow through |
4 | Bio-Scale CHT10I | 10mM KPi(pH7.0),5mM B-ME | 10mM KPi | 2510mM KPi | 4 | 150mM KPi |
5 | Superdex200HR10/30 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | 0.5 | 16ml/24ml | ||
6 | ||||||
7 | ||||||
8 | ||||||
9 |
Products were confirmed by Mass in entire sequence. |
2011.10.20
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