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Available from Riken BioResource Center.

Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST1746
Products aminopeptidase
Plasmid ID 556
Vector  
Purification Lot ST1746
Purified Date 2004.5.13
Protein conc. (mg/ml) 1.74
Total volume (ml) 12
Total protein (mg) 20.9

Culture Lot:

date host cell scale label weight of bacteira (g)
        14.2

Cell Lysis and Heat Treatment:

 
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Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Phe 6ml 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS 1.5M AS 0M AS 6 0 M AS
2 Resource Iso 6ml 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS 1.5M AS 0M AS 6 0.8M AS
3 BioAssist Q 10x10 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 1M NaCl 2.5 0.18M NaCl
4 HiLoad 16/60 Superdex 75 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl        
5 HiPrep 26/10 Desalting 53ml 20mM Tris-HCl(pH8.0),5mM B-ME        
6            
7            
8            
9            

Comment:

Products was confirmed by MS. Some contamination was detected.

2011.10.20

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