| Species | Sulfolobus tokodaii |
|---|---|
| Locus tag | ST1686 |
| Products | N-methylhydantoinase A |
| Plasmid ID | 105 |
| Vector | |
| Purification Lot | ST1686_1 |
| Purified Date | 2004.6.2- |
| Protein conc. (mg/ml) | 3.35 |
| Total volume (ml) | 1.25 |
| Total protein (mg) | 4.18 |
| date | host cell | scale | label | weight of bacteira (g) |
|---|---|---|---|---|
| 23.5 |
|
| Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
|---|---|---|---|---|---|---|
| 1 | Resource Iso 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS | 1.5M AS | 0M AS | 6 | 0.6M AS |
| 2 | BioAssist Q 10x10 | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 1M NaCl | 3 | 0.57M NaCl |
| 3 | Bio-Scale CHT10I | 10mM KPi(pH7.0),5mM B-ME | 10mM KPi | 500mM KPi | 3 | 147mM KPi |
| 4 | Superdex200HR/30 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | ||||
| 5 | HiPrep 26/10 Desalting 53ml | 20mM Tris-HCl(pH8.0),5mM B-ME | ||||
| 6 | ||||||
| 7 | ||||||
| 8 | ||||||
| 9 |
| Products was confirmed by MS. |
2011.10.20
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