Species | Sulfolobus tokodaii |
---|---|
Locus tag | ST1531 |
Products | pyruvate ferredoxin oxidoreductase, gamma subunit |
Plasmid ID | R0232 |
Vector | |
Purification Lot | ST1531 |
Purified Date | 2006.6.19-6.28 |
Protein conc. (mg/ml) | 13.26 |
Total volume (ml) | 0.75 |
Total protein (mg) | 10.4 |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
81.5 |
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | Resource Iso 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,2.0M AS | ,2.0M AS | 0M AS | 5 | 1.3M AS |
2 | Resource Q 6 ml | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 0.6M NaCl | 5 | 180mM NaCl |
3 | HiLoad 16/60 Superdex 75 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | 1 | 83ml/120ml | ||
4 | ||||||
5 | ||||||
6 | ||||||
7 | ||||||
8 | ||||||
9 |
Products was confirmed by Trp-MS. |
2011.10.20
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