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Available from Riken BioResource Center.

Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST1501
Products argininosuccinate synthase
Plasmid ID R0096
Vector  
Purification Lot ST1501_1_2
Purified Date 2006.1.24-2.9
Protein conc. (mg/ml) 8.82
Total volume (ml) 0.96
Total protein (mg) 8.47

Culture Lot:

date host cell scale label weight of bacteira (g)
        66.3

Cell Lysis and Heat Treatment:

26.3 g of E. coli.
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100ml of 20mM Tris-HCl (pH8.0), 5mM B-ME, 50mM NaCl.
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Heat 15 min, 75 deg C.
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Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Iso 6ml 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS 1.5M AS 0M AS 5 0.9M AS
2 Resource Q 6 ml 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 0.5M NaCl 4 0.05M NaCl
3 Bio-Scale CHT5I 10mM KPi(pH7.0),5mM B-ME 10mM KPi 375mM KPi 1 227mM KPi
4 HiLoad 16/60 Superdex 75 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl     1 56mL/120mL
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Comment:

Products was confirmed by Trp-MS.

2011.10.20

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