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Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST1462
Products imidazoleglycerol-phosphate synthase cyclase
Plasmid ID 0088
Vector  
Purification Lot ST1462
Purified Date  
Protein conc. (mg/ml)  
Total volume (ml)  
Total protein (mg)  

Culture Lot:

date host cell scale label weight of bacteira (g)
        20.0

Cell Lysis and Heat Treatment:

 
 
 
 
 
 
 
 
 

Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Phe 6ml 20mM Tris-HCl(pH8.0),5mM B-ME,2.0M AS 2.0M AS 0M AS 5 1.5M AS
2 BioAssist Q 10x10 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 1M NaCl 4 0.1M NaCl
3 G200SW(1inchx60) 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl     2  
4            
5            
6            
7            
8            
9            

Comment:

 

2011.10.20

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