Species | Sulfolobus tokodaii |
---|---|
Locus tag | ST1241 |
Products | aspartate kinase |
Plasmid ID | 0071 |
Vector | |
Purification Lot | ST1241_1a |
Purified Date | |
Protein conc. (mg/ml) | 2.4 |
Total volume (ml) | 0.625 |
Total protein (mg) | 1.5 |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | Resource Phe 6 mL | 20 mM Tris-HCl (pH8.0), 5 mM b-ME, 1.50 M AS | 1.50 M AS | 0 M AS | 6 | 0 M AS |
2 | Resource Q 6 mL | 20 mM Tris-HCl (pH8.0), 5 mM b-ME | 0M NaCl | 2M NaCl | 6 | 192 mM NaCl |
3 | Bio-Scale CHT2I | 10 mM KPi (pH 7.0), 5 mM b-ME | 10mM KPi | 500mM KPi | 1 | 144 mM KPi |
4 | Superdex 200 10/300 GL | 20 mM Tris-HCl (pH8.0), 5 mM b-ME, 150 mM NaCl | 0.5 | |||
5 | HiPrep 26/10 Desalting 53ml | 20 mM Tris-HCl (pH8.0), 5 mM b-ME | ||||
6 | ||||||
7 | ||||||
8 | ||||||
9 |
Precipitated pellet at 1.50 M AS was mixed at purification step 2 after desalting. |
2011.10.20
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