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Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST1215
Products hypothetical cell division control protein
Plasmid ID 0342-5-a
Vector  
Purification Lot ST1215_5-b
Purified Date 040318
Protein conc. (mg/ml) 7.6
Total volume (ml) 0.5
Total protein (mg) 3.8

Culture Lot:

date host cell scale label weight of bacteira (g)
         

Cell Lysis and Heat Treatment:

 
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Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Q 6 mL 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 1M NaCl 5 0M NaCl
2 Resource S 6 mL 50mM MES(pH6.0),5mM B-ME 0M NaCl 1M NaCl 6 0.1M NaCl
3 Bio-Scale CHT10I 10 mM KPi (pH7.0), 5 mM b-ME 10mM KPi 10mM KPi 3 170mM KPi
4 Resource Q 6 mL 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 0.5M NaCl 6 0.2M NaCl
5 BioAssist S 10x10 50mM MES(pH6.0),5mM B-ME 0M NaCl 0.3M NaCl 3 0.18M NaCl
6 Superdex200HR/30 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl     0.5  
7            
8            
9            

Comment:

 

2011.10.20

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