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Available from Riken BioResource Center.

Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST1127
Products hypothetical sulfur oxygenase reductase
Plasmid ID 339
Vector  
Purification Lot ST1127_1
Purified Date  
Protein conc. (mg/ml)  
Total volume (ml)  
Total protein (mg)  

Culture Lot:

date host cell scale label weight of bacteira (g)
        39.1

Cell Lysis and Heat Treatment:

 
 
 
 
 
 
 
 
 

Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Phe 6 mL 20mM Tris-HCl(pH8.0),5mM B-ME,600mM AS 600mM AS 0M AS   600mM AS
2 Resource Iso 6 mL 20mM Tris-HCl(pH8.0),5mM B-ME,600mM AS 600mM AS 0M AS   600mM AS
3 Resource Q 6 mL 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 1M NaCl   M NaCl
4            
5            
6            
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9            

Comment:

 

2011.10.20

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