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Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST1096
Products hypothetical transposase
Plasmid ID 0198
Vector  
Purification Lot ST1096_1
Purified Date 2004.
Protein conc. (mg/ml) 17.3
Total volume (ml) 0.87
Total protein (mg) 15

Culture Lot:

date host cell scale label weight of bacteira (g)
        30

Cell Lysis and Heat Treatment:

 
 
 
 
 
 
 
 
 

Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Phe 6mL 20mM Tris-HCl(pH8.0),5mM B-ME,2.0M AS 2.0M AS 0M AS 5 1.46M AS
2 HiTrap Heparin HP 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 1.2M NaCl 3 850mM NaCl
3 HiLoad 16/60 Superdex 75 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl     1 63ml
4 HiPrep 26/10 Desalting 53ml 20mM Tris-HCl(pH8.0),5mM B-ME        
5            
6            
7            
8            
9            

Comment:

Products was confirmed by MS.

2011.10.20

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