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Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST0960
Products threonine synthase
Plasmid ID 0334
Vector  
Purification Lot ST0960
Purified Date 2003.7.3
Protein conc. (mg/ml) 7.0
Total volume (ml) 2.0
Total protein (mg) 13.8

Culture Lot:

date host cell scale label weight of bacteira (g)
         

Cell Lysis and Heat Treatment:

 
 
 
 
 
 
 
 
 

Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Bio-Scale CHT 10I       4  
2 Resource Iso 6mL 20mM Tris-HCl(pH8.0),5mM B-ME,1.05M AS     4  
3 Resource Phe 6mL       4  
4 BioAssist S 10X10       3  
5 Gel filtration column 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl        
6            
7            
8            
9            

Comment:

 

2011.10.20

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