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Available from Riken BioResource Center.

Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST0827
Products hypothetical protein
Plasmid ID 0434
Vector  
Purification Lot ST0827_4
Purified Date 2004.8.20
Protein conc. (mg/ml) 15
Total volume (ml) 0.022
Total protein (mg) 0.33

Culture Lot:

date host cell scale label weight of bacteira (g)
        65

Cell Lysis and Heat Treatment:

 
 
 
 
 
 
 
 
 

Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Phe 6mL 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS 1.5M AS 0M AS   360mM AS
2 Resource Q 6mL 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 1M NaCl   230mM NaCl
3 Mono Q HR 10/10 20mM Tris-HCl(pH8.0),5mM B-ME     1.5 260mM NaCl
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Comment:

 

2011.10.20

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