Prev.
Available from Riken BioResource Center.

Plasmid & Protein Purification Database -Purification-

Species  
Locus tag ST0724
Products  
Plasmid ID 0430
Vector  
Purification Lot ST0724
Purified Date 2006.7.13
Protein conc. (mg/ml) 44.13
Total volume (ml) 0.41
Total protein (mg) 18.10

Culture Lot:

date host cell scale label weight of bacteira (g)
        40.7

Cell Lysis and Heat Treatment:

40.7 g of E. coli.
↓
100 ml of 20mM Tris-HCl (pH8.0), 5mM B-ME, 50mM NaCl.
↓
 
↓
 
↓
 
↓
 
↓
 
↓
 
 

Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Iso 6mL 20mM Tris-HCl(pH8.0),5mM B-ME,2.0M AS 2.0M AS 0M AS 1 1.3M AS
2 Resource Q 6mL 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 600mM NaCl 4 120mM NaCl
3 HiLoad 16/60 Superdex 200 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl     1 73ml/120ml
4            
5            
6            
7            
8            
9            

Comment:

Products were confirmed by Trypsin MASS.

2011.10.20

- kuramitsuxxxx_html.pl