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Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST0623
Products aminopeptidase
Plasmid ID 0549
Vector  
Purification Lot ST0623_2
Purified Date 2004.8.20
Protein conc. (mg/ml) 1.5
Total volume (ml)  
Total protein (mg) 3

Culture Lot:

date host cell scale label weight of bacteira (g)
        30.3

Cell Lysis and Heat Treatment:

 
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Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Phe 6mL 20mM Tris-HCl(pH8.0),5mM B-ME,0.6M AS 0.6M AS 0M AS 6 240mM AS
2 BioAssist Q 10X10 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 1M NaCl 3 320mM NaCl
3 HiLoad 16/60 Superdex 200 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl        
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Comment:

 

2011.10.20

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