Species | |
---|---|
Locus tag | ST0600 |
Products | |
Plasmid ID | 0032 |
Vector | |
Purification Lot | ST0600 |
Purified Date | 2004.10.14 |
Protein conc. (mg/ml) | 11.44 |
Total volume (ml) | 3.75 |
Total protein (mg) | 42.9 |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
26.7 |
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | Resource Phe 6mL | 20mM Tris-HCl(pH8.0),5mM B-ME,1.35M AS | 1.35M AS | 0M AS | 6 | 930mM AS |
2 | BioAssist S 10X10 | 50mM MES(pH6.0),5mM B-ME | 0M NaCl | 1M NaCl | 2 | 450mM NaCl |
3 | HiLoad 16/60 Superdex 75 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | 0.5 | 72ml | ||
4 | ||||||
5 | ||||||
6 | ||||||
7 | ||||||
8 | ||||||
9 |
Products were confirmed by Trypsin-Mass MSCOT. |
2011.10.20
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