Species | |
---|---|
Locus tag | ST0385 |
Products | |
Plasmid ID | 0019 |
Vector | |
Purification Lot | ST0385_1a |
Purified Date | |
Protein conc. (mg/ml) | |
Total volume (ml) | |
Total protein (mg) |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
14.2 |
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | Resource Iso 6mL | 1.5M AS | 0M AS | 5 | M AS | |
2 | BioAssist Q 10X10 | 0M NaCl | 500mM NaCl | 5 | ||
3 | Superdex 200 HR1 0/30 | |||||
4 | Bio-Scale CHT10I | 10mM KPi(pH7.0),5mM B-ME | 10mM KPi | 250mM KPi | 4 | |
5 | ||||||
6 | ||||||
7 | ||||||
8 | ||||||
9 |
At the end of purification 2, solution was divided into three fractions, a, b, and c, and the purified individually. |
2011.10.20
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