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Available from Riken BioResource Center.

Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST0364
Products conserved hypothetical protein
Plasmid ID 0576
Vector  
Purification Lot ST0364_1
Purified Date 2005.3.3-3.14
Protein conc. (mg/ml) 15.2
Total volume (ml) 0.55
Total protein (mg) 8.36

Culture Lot:

date host cell scale label weight of bacteira (g)
        60

Cell Lysis and Heat Treatment:

30.0 g of E. coli. Two times.
 
 
 
 
 
 
 
 

Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Phe 6ml 50mM Tris-HCl(pH9.5),5mM B-ME,0.7M AS 0.7M AS 0M AS 5 0.34M AS
2 Resource Q 6 ml 50mM Tris-HCl(pH9.51),5mM B-ME 0M NaCl 0.3M NaCl 5 185mM NaCl
3 Resource S 6 ml 50mM MES(pH6.5),5mM B-ME 0M NaCl 0.3M NaCl 5 70mM NaCl
4 HiLoad 16/60 Superdex200 50mM Tris-HCl(pH9.5),5mM B-ME,500mM NaCl     1 87.9ml
5 HiPrep 26/10 Desalting 53ml 50mM CAPS(pH10.5),5mM B-ME        
6            
7            
8            
9            

Comment:

Products were confirmed by MS. Stored as glycerol stock.

2011.10.20

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