| Species | Sulfolobus tokodaii |
|---|---|
| Locus tag | ST0345 |
| Products | hypothetical peptidyl prolyl cis-trans isomerase |
| Plasmid ID | 407 |
| Vector | |
| Purification Lot | ST0345_1 |
| Purified Date | 2004.10.6 |
| Protein conc. (mg/ml) | 13.7 |
| Total volume (ml) | 0.8 |
| Total protein (mg) | 11.0 |
| date | host cell | scale | label | weight of bacteira (g) |
|---|---|---|---|---|
| 40 |
|
ST0345.pdf |
| Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
|---|---|---|---|---|---|---|
| 1 | Resource Phe 6mL | 20mM Tris-HCl(pH8.0),5mM B-ME,1.35M AS | 1.35M AS | 0M AS | 6 | 994mM AS |
| 2 | Resource Q 6mL | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 2M NaCl | 6 | 80mM NaCl |
| 3 | Bio-Scale CHT10I | 10mM KPi(pH7.0),5mM B-ME | 10mM KPi | 500mM KPi | 2 | 98.2mM KPi |
| 4 | Resource S 6mL | 50mM MES(pH5.7),5mM B-ME | 0M NaCl | 2M NaCl | 6 | 142mM NaCl |
| 5 | HiLoad 16/60 Superdex 75 | 20mM Tris-HCl(pH8.0),5mM B-ME | 1 | 45min | ||
| 6 | ||||||
| 7 | ||||||
| 8 | ||||||
| 9 |
2011.10.20
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