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Plasmid & Protein Purification Database -Purification-

Species  
Locus tag ST0296
Products  
Plasmid ID 0012
Vector  
Purification Lot ST0296_1a
Purified Date 2004.3.25
Protein conc. (mg/ml) 6.15
Total volume (ml) 1.5
Total protein (mg) 9.2

Culture Lot:

date host cell scale label weight of bacteira (g)
        10

Cell Lysis and Heat Treatment:

 
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Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Iso 6mL 20mM Tris-HCl(pH8.0),5mM B-ME,1.35M AS 1.35M AS 0M AS 6 between 300mM AS and 700mM AS
2 Resource Q 6mL 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 1M NaCl 6 between200mM NaCl and 300mM NaCl
3 BioAssist Q 10X10 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 300mM NaCl 3 between200mM NaCl and 250mM NaCl
4 Bio-Scale CHT10I 10mM KPi(pH7.0),5mM B-ME   500mM KPi 3 between200mM KPi and 250mM KPi
5 HiLoad 16/60 Superdex75 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl     0.5 96min
6            
7            
8            
9            

Comment:

At the end of Purification 4, solution was divided two groupes, 1a and 1b. Products were confirmed by Mass.

2011.10.20

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