| Species | |
|---|---|
| Locus tag | ST0282 |
| Products | |
| Plasmid ID | 568 |
| Vector | |
| Purification Lot | ST0282_2 |
| Purified Date | |
| Protein conc. (mg/ml) | 17.3 |
| Total volume (ml) | 0.96 |
| Total protein (mg) | 16.6 |
| date | host cell | scale | label | weight of bacteira (g) |
|---|---|---|---|---|
| 60 |
|
| Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
|---|---|---|---|---|---|---|
| 1 | Resource Iso 6mL | 20mM Tris-HCl(pH8.0),5mM B-ME,1.0M AS | 1M AS | 0M AS | 6 | between 120mM AS and 400mM AS |
| 2 | Mono Q HR 10/10 | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 2M NaCl | 0.5 | between 40mM NaCl and 80mM NaCl |
| 3 | Bio-Scale CHT10I | 10mM KPi(pH7.0),5mM B-ME | 500mM KPi | 3 | 70mM KPi | |
| 4 | HiLoad 16/60 Superdex 75 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | ||||
| 5 | HiPrep 26/10 Desalting 53ml | 20mM Tris-HCl | ||||
| 6 | ||||||
| 7 | ||||||
| 8 | ||||||
| 9 |
| Se-Met protein. |
2011.10.20
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