| Species | |
|---|---|
| Locus tag | ST0282 |
| Products | |
| Plasmid ID | 568 |
| Vector | |
| Purification Lot | ST0282_1 |
| Purified Date | 2004.6.25 |
| Protein conc. (mg/ml) | 17.1 |
| Total volume (ml) | 2.0 |
| Total protein (mg) | 34.14 |
| date | host cell | scale | label | weight of bacteira (g) |
|---|---|---|---|---|
| 26.8 |
|
| Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
|---|---|---|---|---|---|---|
| 1 | Resource Iso 6mL | 20mM Tris-HCl(pH8.0),5mM B-ME,1.0M AS | 1M AS | 0M AS | 6 | 700mM AS |
| 2 | BioAssist Q 10X10 | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 1M NaCl | 3 | 20mM NaCl |
| 3 | Bio-Scale CHT10I | 10mM KPi(pH7.0),5mM B-ME | 10mM KPi | 500mM KPi | 3 | 70mM KPi |
| 4 | Superdex 200 HR 10/30 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | ||||
| 5 | HiPrep 26/10 Desalting 53ml | 20mM Tris-HCl(pH8.0),5mM B-ME,40mM NaCl | ||||
| 6 | ||||||
| 7 | ||||||
| 8 | ||||||
| 9 |
| To avoid precipitation, a little amount of solt is required. |
2011.10.20
- kuramitsuxxxx_html.pl