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Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST0242
Products phosphomannomutase
Plasmid ID 0153
Vector  
Purification Lot ST0242_2
Purified Date 2004.11.11
Protein conc. (mg/ml) 14.6
Total volume (ml) 3.74
Total protein (mg) 54.5

Culture Lot:

date host cell scale label weight of bacteira (g)
        16

Cell Lysis and Heat Treatment:

 
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Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Phe 6mL 20mM Tris-HCl(pH8.0),5mM B-ME,1.35M AS 1.35M AS 0M AS 5 760mM AS
2 Resource Q 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 500mM NaCl 5 50mM NaCl
3 Resource S 6mL 50mM MES(pH5.6),5mM B-ME 0M NaCl 500mM NaCl 5 50mM NaCl
4 Resource Phe 6mL 20mM Tris-HCl(pH8.0),5mM B-ME,1.35M AS 1.35M AS 0M AS 5 760mM AS
5 HiLoad 16/60 Superdex 200 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl     1 63ml
6 HiPrep 26/10 Desalting 53ml 20mM Tris-HCl(pH8.0),5mM B-ME        
7            
8            
9            

Comment:

 

2011.10.20

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