Step |
Column |
Buffer |
Gradient 1 |
Gradient 2 |
FlowRate (ml/min) |
Eluted |
1 |
Resource Phe 6mL |
20mM Tris-HCl(pH7.8),5mM B-ME,1.35M AS |
1.35M AS |
0M AS |
5 |
500mM AS |
2 |
Resource Q |
20mM Tris-HCl(pH7.8),5mM B-ME |
0M NaCl |
1M NaCl |
6 |
400mM NaCl |
3 |
BioAssist Q 10X10 |
20mM Tris-HCl(pH7.8),5mM B-ME |
0M NaCl |
1M NaCl |
3 |
400mM NaCl |
4 |
Bio-Scale CHT 10I |
10mM KPi(pH7.0),5mM B-ME |
10mM KPi |
500mM KPi |
3 |
80mM KPi |
5 |
Bioassist S 10X10 |
20mM MES(pH5.8),5mM B-ME |
0M NaCl |
400mM NaCl |
2 |
10mM NaCl |
6 |
HiLoad 16/60 Superdex75 |
20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl |
|
|
0.9 |
|
7 |
HiTrap heparin HP |
20mM MES(pH5.8),5mM B-ME |
0M NaCl |
500mM NaCl |
2.5 |
100mM NaCl |
8 |
:HiPrep 26/10 Desalting 53ml |
20mM Tris-HCl(pH7.8),5mM B-ME |
|
|
|
|
9 |
|
|
|
|
|
|