| Species | |
|---|---|
| Locus tag | ST0223 |
| Products | |
| Plasmid ID | 0303 |
| Vector | |
| Purification Lot | ST0223_1 |
| Purified Date | 2004.11.2 |
| Protein conc. (mg/ml) | |
| Total volume (ml) | |
| Total protein (mg) |
| date | host cell | scale | label | weight of bacteira (g) |
|---|---|---|---|---|
| 30 |
|
| Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
|---|---|---|---|---|---|---|
| 1 | Resource Phe 6mL | 20mM Tris-HCl(pH8.0),5mM B-ME,1.35M AS | 1.35M AS | 0M AS | 5 | 500mM AS |
| 2 | Resource Q 6mL | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 600mM NaCl | 5 | 180mM NaCl |
| 3 | HiTrap Heparin HP | 50mM MES(pH6.0),5mM B-ME | 0M NaCl | 800mM NaCl | 5 | 280mM NaCl |
| 4 | BioAssist Q 10X10 | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 400mM NaCl | 2 | 240mM NaCl |
| 5 | HiLoad 16/60 Superdex200 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | 1 | 75ml | ||
| 6 | ||||||
| 7 | ||||||
| 8 | ||||||
| 9 |
| Final products of the lot 1 and 2 were mixed together. |
2011.10.20
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