Species | |
---|---|
Locus tag | ST0212 |
Products | |
Plasmid ID | 0149 |
Vector | |
Purification Lot | ST0212_3 |
Purified Date | 2004.9.13 |
Protein conc. (mg/ml) | 22 |
Total volume (ml) | 0.72 |
Total protein (mg) | 15.8 |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
15 |
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | Resource Iso 6mL | 20mM Tris-HCl(pH8.0),5mM B-ME,1.08M AS | 1.08M AS | 0M AS | 5 | 600mM AS |
2 | Resource Q 6mL | 20mM Tris-HCl(pH9.0),5mM B-ME | 0M NaCl | 500mM NaCl | 5 | 150mM NaCl |
3 | HiTrap Heparin HP | 50mM MES(pH6.0),5mM B-ME,120mM NaCl | 120mM NaCl | 800mM NaCl | 3 | 460mM NaCl |
4 | HiLoad 16/60 Superdex75 | 50mM Tris-HCl(pH9.0),5mM B-ME,150mM NaCl | 1 | |||
5 | ||||||
6 | ||||||
7 | ||||||
8 | ||||||
9 |
It was hard to solve this solution at less than 110 mM NaCl or less than pH9.0. Final products of the lot 2 and 3 were mixed together. Solution was yellow. |
2011.10.20
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