Species | |
---|---|
Locus tag | ST0079 |
Products | |
Plasmid ID | 0522 |
Vector | |
Purification Lot | ST0079_1b |
Purified Date | 2005.3.1 |
Protein conc. (mg/ml) | 0.42 |
Total volume (ml) | 3 |
Total protein (mg) | 1.26 |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
34.0 |
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | Resource Phe 6mL | 20mM Tris-HCl(pH8.0),5mM B-ME,1.05M AS | 1.05M AS | 0M AS | 2 | 0M AS |
2 | Resource Iso 6mL | 20mM Tris-HCl(pH8.0),5mM B-ME,1.20M AS | 1.20M AS | 0M AS | 3 | 900mM AS(between 1.0M AS and 660mM AS) |
3 | Resource Q 6mL | 20mM Tris-HCl(pH8.0),5mM B-ME | 0mM NaCl | 500mM NaCl | 3 | 240mM NaCl |
4 | Bio-Scale CHT10I | 10mM KPi(pH7.0),5mM B-ME | 10mM KPi | 500mM KPi | 2 | between 120mM KPi and 320mM KPi |
5 | Resource Q 6mL | 20mM Tris-HCl(pH8.0),5mM B-ME | 0mM NaCl | 500mM NaCl | 3 | 280mM NaCl |
6 | ||||||
7 | ||||||
8 | ||||||
9 |
At the end of Purification 4, solution was divided two groupes, 1a and 1b. Products of the main band (43kDa), 35 kDa and 31 kDa (C terminal half) were confirmed by Trp-Mass. |
2011.10.20
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