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Available from Riken BioResource Center.

Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST0043
Products hypothetical transposase
Plasmid ID 0133
Vector  
Purification Lot ST0043_1a
Purified Date 2006.9.27
Protein conc. (mg/ml) 9.05
Total volume (ml) 0.85
Total protein (mg) 7.69

Culture Lot:

date host cell scale label weight of bacteira (g)
        38.6

Cell Lysis and Heat Treatment:

38.6 g of E. coli cells
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200 ml of 20 mM Tris-HCl (pH 8.0), 5 mM B-ME, 50 mM NaCl.
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Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Iso 6mL 20 mM Tris-HCl (pH8.0), 5 mM b-ME, 2.2 M AS 2.2 M AS 0M NaCl 4 1.9 M AS
2 Resource S 6mL 20 mM Tris-HCl (pH8.0), 5 mM b-ME 0M NaCl 500mM NaCl 5 360mM NaCl
3 HiLoad 16/60 Superdex 75 20 mM Tris-HCl (pH8.0), 5 mM b-ME, 150 mM NaCl     1 71mL/120mL
4            
5            
6            
7            
8            
9            

Comment:

Products was confirmed by MS. Products of 16K and 32K were identified as objective protein by Trp-MS. This lot (1a) was purified from a peak appeared in the step of Purification 3.

2011.10.20

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