Species | |
---|---|
Locus tag | APE0550 |
Products | |
Plasmid ID | R4333 |
Vector | |
Purification Lot | APE0550_9 |
Purified Date | 2006.5.31-6.12 |
Protein conc. (mg/ml) | |
Total volume (ml) | |
Total protein (mg) |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
165 |
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | Resource Iso 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,1.65M AS | 1.65M AS | 0M AS | 5 | |
2 | Resource Q 6 ml | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 0.6M NaCl | 4 | |
3 | HiLoad 16/60 Superdex200 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | ||||
4 | ||||||
5 | ||||||
6 | ||||||
7 | ||||||
8 | ||||||
9 |
2011.10.20
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