| Species | |
|---|---|
| Locus tag | APE0550 |
| Products | |
| Plasmid ID | R4333 |
| Vector | |
| Purification Lot | APE0550_4 |
| Purified Date | 2006.2.21-2.24 |
| Protein conc. (mg/ml) | 8.02 |
| Total volume (ml) | 1.3 |
| Total protein (mg) | 10.4 |
| date | host cell | scale | label | weight of bacteira (g) |
|---|---|---|---|---|
| 45.2 |
|
| Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
|---|---|---|---|---|---|---|
| 1 | Resource Iso 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,1.75M AS | 1.8M AS | 0M AS | 4 | 1.45M AS |
| 2 | Resource S 6 ml | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 2M NaCl | 4 | 0.36M NaCl |
| 3 | ||||||
| 4 | ||||||
| 5 | ||||||
| 6 | ||||||
| 7 | ||||||
| 8 | ||||||
| 9 |
| Products were comfirmed by MS. Purification was abandoned after ResourceS column step taking yeald of proteins and conposition of buffers into consideration. |
2011.10.20
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