| Species | |
|---|---|
| Locus tag | APE0457 |
| Products | |
| Plasmid ID | 4018 |
| Vector | |
| Purification Lot | APE0457_1b |
| Purified Date | 2004.11.26- |
| Protein conc. (mg/ml) | 0.85 |
| Total volume (ml) | 1.5 |
| Total protein (mg) | 1.28 |
| date | host cell | scale | label | weight of bacteira (g) |
|---|---|---|---|---|
| 34.4 |
|
| Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
|---|---|---|---|---|---|---|
| 1 | Resource Phe 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,0.6M AS | 0.6M AS | 0M AS | 6 | 0.6M AS |
| 2 | Resource Q 6 ml | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 2M NaCl | 6 | 312mM NaCl |
| 3 | Bio-Scale CHT2I | 10mM KPi(pH7.0),5mM B-ME | 10mM KPi | 500mM KPi | 1 | 180.5mM KPi |
| 4 | HiLoad 16/60 Superdex 75 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | 1 | 54min | ||
| 5 | ||||||
| 6 | ||||||
| 7 | ||||||
| 8 | ||||||
| 9 |
| Products containing less contaminant from hydroxyapatite column were concentrated with a gel purification. Products were confirmed by MS. |
2011.10.20
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