Species | |
---|---|
Locus tag | APE0405 |
Products | |
Plasmid ID | 4016 |
Vector | |
Purification Lot | APE0405_a |
Purified Date | 2005.10.3-10.11 |
Protein conc. (mg/ml) | 13.44 |
Total volume (ml) | 1.2 |
Total protein (mg) | 16.13 |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
20.6 |
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | Resource Iso 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS | 1.5M AS | 0M AS | 4 | 0.8M AS |
2 | Resource Q 6 ml | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 1M NaCl | 4 | 0.27M NaCl |
3 | HiLoad 16/60 Superdex 75 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | 1 | 65min | ||
4 | ||||||
5 | ||||||
6 | ||||||
7 | ||||||
8 | ||||||
9 |
Products containing less contaminant from Reso Q column were concentrated with a gel purification. |
2011.10.20
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