Species | |
---|---|
Locus tag | APE0319 |
Products | |
Plasmid ID | R4703 |
Vector | |
Purification Lot | APE0319_a |
Purified Date | |
Protein conc. (mg/ml) | 12.68 |
Total volume (ml) | 1.50 |
Total protein (mg) | 19.0 |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
28.3 |
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | Resource Iso 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS | 1.5M AS | 0M AS | 3 | 1.17M AS |
2 | Resource Q 6 ml | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 2M NaCl | 4 | 0.09M NaCl |
3 | HiLoad 16/60 Superdex200 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | 1 | 80min | ||
4 | ||||||
5 | ||||||
6 | ||||||
7 | ||||||
8 | ||||||
9 |
Maltimeric products. Because two peaks were obtaied at the step of Reso Q, products were gel purified independent two (a and b) ways. Products were eluted at 80 min in a procedure and at 68 min in b procedure. Products were able to purified without precipitation in the solution. Products of high molecular weight were also confirmed. |
2011.10.20
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