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Available from Riken BioResource Center.

Plasmid & Protein Purification Database -Purification-

Species  
Locus tag APE0091
Products  
Plasmid ID R4234
Vector  
Purification Lot APE0091
Purified Date  
Protein conc. (mg/ml)  
Total volume (ml)  
Total protein (mg)  

Culture Lot:

date host cell scale label weight of bacteira (g)
        24.0

Cell Lysis and Heat Treatment:

24 g of E. coli.
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100 ml of 20mM Tris-HCl (pH8.0), 5mM B-ME, 150mM NaCl.
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Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Phe 6ml 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS 1.5M AS 0M AS 2.5 1.33M AS
2 Resource Q 6 ml          
3            
4            
5            
6            
7            
8            
9            

Comment:

Purified products were not target protein.

2011.10.20

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