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Akihiko Nakano's Resource

Akihiko Nakano's web page in English    in Japanese.

Learn more about their research in Reseachmap in English    in Japanese.
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Study in membrane trafficking of budding yeast, S. cereviciae.

Publication history

PMIDTITLELink
27445311Ishii M, Suda Y, Kurokawa K, Nakano A. (2016) COPI is essential for Golgi cisternal maturation and dynamics. J. Cell Sci. 129, pp3251-3261.CLICK to check clones!
19170767Murakami-Sekimata A, Sato K, Sato K, Takashima A, Nakano A. (2009) O-Mannosylation is required for the solubilization of heterologously expressed human beta-amyloid precursor protein in Saccharomyces cerevisiae. Genes Cells 14, pp205-215.CLICK to check clones!
18343875Takeda Y, Nakano A. (2008) In vitro formation of a novel type of membrane vesicles containing Dpm1p: putative transport vesicles for lipid droplets in budding yeast. J Biochem. 143, pp803-811.CLICK to check clones!
17973654Higashio H1, Sato K, Nakano A. (2008) Smy2p participates in COPII vesicle formation through the interaction with Sec23p/Sec24p subcomplex. Traffic. 9, pp79-93.CLICK to check clones!
16735437Yahara N, Sato K, Nakano A. (2006) The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. J Cell Sci. 15, pp2604-2612.CLICK to check clones!
16699523Matsuura-Tokita K, Takeuchi M, Ichihara A, Mikuriya K, Nakano A. (2006) Live imaging of yeast Golgi cisternal maturation. Nature 441, pp1007-1010CLICK to check clones!
15665868Sato K, Nakano A. (2005) Dissection of COPII subunit-cargo assembly and disassembly kinetics during Sar1p-GTP hydrolysis. Nat Struct Mol Biol. 12, pp167-174.CLICK to check clones!
14699055Sato M, Sato K, Nakano A. (2004) Endoplasmic reticulum quality control of unassembled iron transporter depends on Rer1p-mediated retrieval from the golgi. Mol Biol Cell. 15, pp1417-1424.CLICK to check clones!
14627716Sato K, Nakano A.(2004) Reconstitution of coat protein complex II (COPII) vesicle formation from cargo-reconstituted proteoliposomes reveals the potential role of GTP hydrolysis by Sar1p in protein sorting. J Biol Chem. 279, pp1330-1335.CLICK to check clones!
12972550Sato K, Sato M, Nakano A. (2003) Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol Biol Cell. 14, pp3605-3616.CLICK to check clones!
12857885Sato K, Nakano A. (2003) Oligomerization of a cargo receptor directs protein sorting into COPII-coated transport vesicles. Mol Biol Cell. 14, pp3055-3063.CLICK to check clones!
12810702Umebayashi K, Nakano A. (2003) Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J Cell Biol. 161, pp1117-1131CLICK to check clones!
12134087Sato K, Nakano A. (2002) Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae. Mol Biol Cell. 13, pp2518-2532.CLICK to check clones!
11442630Sato M, Fujisaki S, Sato K, Nishimura Y, Nakano A. (2001) Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles in dolichol synthesis. Genes Cells. 6, pp495-506.CLICK to check clones!
11238450Sato K, Sato M, Nakano A. (2001) Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer. J Cell Biol. 152, pp935-944CLICK to check clones!
11168590Saito-Nakano Y, Nakano A. (2000) Sed4p functions as a positive regulator of Sar1p probably through inhibition of the GTPase activation by Sec23p. Genes Cells. 5, pp1039-1048.CLICK to check clones!
11160834Yahara N, Ueda T, Sato K, Nakano A. (2001) Multiple roles of Arf1 GTPase in the yeast exocytic and endocytic pathways. Mol Biol Cell. 12, pp221-238.CLICK to check clones!
11044978Hopkins BD, Sato K, Nakano A, Graham TR. (2000) Introduction of Kex2 cleavage sites in fusion proteins for monitoring localization and transport in yeast secretory pathway. Methods Enzymol. 327, pp107-118.CLICK to check clones!
10791890Matsuda N, Ueda T, Sasaki Y, Nakano A. (2000) Overexpression of PRA2, a Rab/Ypt-family small GTPase from Pea Pisum sativum, aggravates the growth defect of yeast ypt mutants. Cell Struct Funct. 25, pp11-20.CLICK to check clones!
10788782Homma K, Yoshida Y, Nakano A. (2000) Evidence for recycling of cytochrome P450 sterol 14-demethylase from the cis-Golgi compartment to the endoplasmic reticulum (ER) upon saturation of the ER-retention mechanism. J Biochem. 127, pp747-754.CLICK to check clones!
9920934Murakami A, Kimura K, Nakano A. (1999) The inactive form of a yeast casein kinase I suppresses the secretory defect of the sec12 mutant. Implication of negative regulation by the Hrr25 kinase in the vesicle budding from the endoplasmic reticulum. J Biol Chem. 274, pp3804-3810.CLICK to check clones!
9880808Saito Y, Yamanushi T, Oka T, Nakano A. (1999) Identification of SEC12, SED4, truncated SEC16, and EKS1/HRD3 as multicopy suppressors of ts mutants of Sar1 GTPase. J Biochem. 125, pp130-137.CLICK to check clones!
9858571Sato M, Sato K, Nishikawa S, Hirata A, Kato J, Nakano A. (1999) The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis. Mol Cell Biol. 19, pp471-483.CLICK to check clones!
9756629Saito Y, Kimura K, Oka T, Nakano A. (1998) Activities of mutant Sar1 proteins in guanine nucleotide binding, GTP hydrolysis, and cell-free transport from the endoplasmic reticulum to the Golgi apparatus. J Biochem. 124, pp816-823.CLICK to check clones!
8889833Yamanushi T, Hirata A, Oka T, Nakano A. (1996) Characterization of yeast sar1 temperature-sensitive mutants, which are defective in protein transport from the endoplasmic reticulum. J Biochem. 120, pp452-458.CLICK to check clones!
8707815Sato M, Sato K, Nakano A. (1996) Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain. J Cell Biol. 134,pp279-93CLICK to check clones!
8589449Sato K, Nishikawa S, Nakano A. (1995) Membrane protein retrieval from the Golgi apparatus to the endoplasmic reticulum (ER): characterization of the RER1 gene product as a component involved in ER localization of Sec12p. Mol Biol Cell. 6, pp1459-1477.CLICK to check clones!
8367481Nishikawa S, Nakano A. (1993) Identification of a gene required for membrane protein retention in the early secretory pathway. Proc Natl Acad Sci U S A. 90, pp8179-8183.CLICK to check clones!
7865879Nishikawa S, Hirata A, Nakano A. (1994) Inhibition of endoplasmic reticulum (ER)-to-Golgi transport induces relocalization of binding protein (BiP) within the ER to form the BiP bodies. Mol Biol Cell. 5, pp1129-1143.CLICK to check clones!
7822237Nakano A, Otsuka H, Yamagishi M, Yamamoto E, Kimura K, Nishikawa S, Oka T. (1994) Mutational analysis of the Sar1 protein, a small GTPase which is essential for vesicular transport from the endoplasmic reticulum. J Biochem. 116, pp243-247.CLICK to check clones!
1907491Nishikawa S, Nakano A. (1991) The GTP-binding Sar1 protein is localized to the early compartment of the yeast secretory pathway. Biochim. Biophys. Acta. 1093, pp135-143CLICK to check clones!
2512296Nakano A, Muramatsu M. (1989) A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus. J Cell Biol. 109, pp2677-2691.CLICK to check clones!
3047151Nakano A, Brada D, Schekman R. (1988) A membrane glycoprotein, Sec12p, required for protein transport from the endoplasmic reticulum to the Golgi apparatus in yeast. J Cell Biol. 107, pp851-863.CLICK to check clones!


Clone Table

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COPI is essential for Golgi cisternal maturation and dynamics. (2016) J. Cell Sci. 129, pp.3251-3261. PMID 27445311.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB14670pRS316-GNT1-GFPCENURA3ApPADH1-GNT1-EGFP-TCMK1GFP labeling medial cisternae
RDB14671pRS314-GNT1-GFPCENTRP1ApPADH1-GNT1-EGFP-TCMK1GFP labeling medial cisternae
RDB14672pRS316-SYS1-GFPCENURA3ApPADH1-SYS1-EGFP-TCMK1GFP labeling trans cisternae
RDB14673pRS304-SEC71TMD-GFPInt.TRP1ApPSEC71-SEC71TMD-EGFP-TSEC71GFP labeling ER

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O-Mannosylation is required for the solubilization of heterologously expressed human beta-amyloid precursor protein in Saccharomyces cerevisiae. (2009) Genes Cells 14, pp205-215. PMID 19170767.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08714pTU1-PalphaAPPCENURA3ApPTDH3-sp-APP1(Human)-TCMK1sp: signal peptide from MFalpha1

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In vitro formation of a novel type of membrane vesicles containing Dpm1p: putative transport vesicles for lipid droplets in budding yeast. (2008) J Biochem. 143, pp803-811. PMID 18343875.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08715pYTY102CENURA3ApP(TDH3) DPM1-DPM1-GFP-TCMK1
RDB08716pYTY103CENURA3ApP(ADH1) EGR6-ERG6-mRFP-TCMK1
RDB08717pYTY104CENTRP1ApP(TDH3) EGR6-ERG6-mRFP-TCMK1

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Smy2p participates in COPII vesicle formation through the interaction with Sec23p/Sec24p subcomplex. (2008) Traffic 9, pp79-93. PMID 17973654.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08718pYPL1URA3ApYPL105cSYH1: paralog of SMY2
RDB08719pSMY1URA3ApSMY2
RDB08720pSMY3CENURA3ApSMY2
RDB08721pSMY2URA3ApSMY2-3HA
RDB08722pSMY4CENURA3ApSMY2-3HA
RDB08723pSMY5CENURA3Apsmy2(mutant A)-3HASmy2(W279A Y284A)
RDB08724pSMY6CENURA3Apsmy2(mutant B)-3HASmy2Δ(269-285)
RDB08725pSMY7CENURA3Apsmy2(mutant C)-3HASmy2Δ(420-490)
RDB08726pSMY8CENURA3Apsmy2(mutant D)-3HASmy2Δ(337-790)
RDB08727pSMY9CENURA3Apsmy2(mutant E)-3HASmy2Δ(337-676)
RDB08728pSMY10CENURA3Apsmy2(mutant F)-3HASmy2Δ(337-500)
RDB08729pSMY11CENURA3Apsmy2(mutant G)-3HASmy2Δ(501-676)
RDB08730pSMY12CENURA3Apsmy2(mutant H)-3HASmy2Δ(52-336)
RDB08731pSMY13CENURA3Apsmy2(mutant I)-3HASmy2Δ(52-500)
RDB08732pJG-SEC23HIS3ApPGAL1-B42-HA-SEC23Y2H system (AD)
RDB08733pJG-SEC24HIS3ApPGAL1-B42-HA-SEC24Y2H system (AD)
RDB08734pEG-SEC24TRP1ApPADH1-LexA-SEC24Y2H system (BD)
RDB08735pEG-SMY2TRP1ApPADH1-LexA-SMY2Y2H system (BD)

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The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. (2006) J Cell Sci. 15, pp2604-2612. PMID 16735437.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08549pNY24-GLO3TRP1ApGLO3
RDB08550pNY24-3HA-GLO3TRP1Ap3HA-GLO3
RDB08551pNY24-GCS1TRP1ApGCS1
RDB08552pNY24-GCS1-3HATRP1ApGCS1-3HA
RDB08553pNY24-GLO3-A-GCS1TRP1ApPGLO3-glo3-gcs1Glo3(N-64)-Gcs1(57-C)
RDB08554pNY24-GLO3-N-GCS1TRP1ApPGLO3-glo3-gcs1Glo3(N-122)-Gcs1(117-C)
RDB08555pNY24-GCS1-A-GLO3TRP1ApPGCS1-gcs1-glo3Gcs1(N-60)-Glo3 (62-C)
RDB08556pNY24-GCS1-N-GLO3TRP1ApPGCS1-gcs1-glo3Gcs1(N-111)-Glo3(119-C)
RDB08557pNY24-AGE1TRP1ApAGE1
RDB08558pNY24-AGE2TRP1ApAGE2

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Live imaging of yeast Golgi cisternal maturation. (2006) Nature 441, pp1007-1010. PMID 16699523.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08658pRS316-GFP-SED5-SBCENURA3ApPTDH3-EGFP-SED5-TCMK1
RDB08659pRS316-mRFP-SED5CENURA3ApPTDH3-mRFP-SED5-TCMK1
RDB08660pRS316-GFP-GOS1CENURA3ApPTDH3-EGFP-GOS1-TCMK1
RDB08661pRS316-mRFP-GOS1CENURA3ApPTDH3-mRFP-GOS1-TCMK1
RDB08662pRS316-SEC7-GFPCENURA3ApPADH1-SEC7-EGFP-TCMK1
RDB08663pRS316-SEC7-mRFPCENURA3ApPADH1-SEC7-mRFP-TCMK1
RDB08918pRS316-GFP-RER1-mRFP-SED5CENURA3ApPTDH3-mRFP-SED5-TCMK1 PTDH3-EGFP-RER1-TCMK1
RDB08919pRS314-GFP-RER1-mRFP-GOS1CENTRP1ApPTDH3-EGFP-RER1-TCMK1 PTDH3-mRFP-GOS1-TCMK1
RDB08920pRS316-GFP-RER1-SEC7-mRFPCENURA3ApPTDH3-EGFP-RER1-TCMK1 PADH1-SEC7-mRFP-TCMK1
RDB08921pRS316rev-GFP-SED5-SEC7-mRFPCENURA3ApPTDH3-EGFP-SED5-TCMK1 PADH1-SEC7-mRFP-TCMK1
RDB08922pRS316-mRFP-SED5-SEC7-GFPCENURA3ApPTDH3-mRFP-SED5-TCMK1 PADH1-SEC7-EGFP-TCMK1
RDB08923pRS316rev-mRFP-SED5-SEC7-GFPCENURA3ApPTDH3-mRFP-SED5-TCMK1 PADH1-SEC7-EGFP-TCMK1
RDB08924pRS316-SEC7-mRFP-GFP-GOS1CENURA3ApPADH1-SEC7-mRFP-TCMK1 PTDH3-EGFP-GOS1-TCMK1
RDB08925pRS316rev-GFP-GOS1-SEC7-mRFPCENURA3ApPTDH3-EGFP-GOS1-TCMK1 PADH1-SEC7-mRFP-TCMK1

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Dissection of COPII subunit-cargo assembly and disassembly kinetics during Sar1p-GTP hydrolysis. (2005) Nat Struct Mol Biol. 12, pp167-174. PMID 15665868.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08689pKSY193URA3ApHis6-YFP-SEC24
RDB08690pKSY197CENURA3Ap2xHA-CFP-BET1
RDB08691pKSY198CENURA3ApPTDH3-2xHA-CFP-Strep-SEC22-TCMK1
RDB08692pET21a-SED5bacterialApSed5p (1-316)-His6expression vector (T7 promoter, DE3 required)
RDB08708pKSE165bacterialApStrep2-CFP-Bet1expression vector (tet promoter)
RDB08709pKSE168bacterialApStrep-CFP-Strep-Sec22expression vector (tet promoter)
RDB08710pKSE174bacterialApStrep2-CFP-bet1(L54A E55A)expression vector (tet promoter)
RDB08711pKSE176bacterialApGST-Sec12(1-373)expression vector (tac promoer), Sec12Δluminal-domain
RDB08712pKSE188bacterialCmHis6-Bos1(1-222)expression vector (lac promoter)
RDB08713pKSE203bacterialApMBP-CFP-Ufe1expression vector (tac promoter)

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Endoplasmic reticulum quality control of unassembled iron transporter depends on Rer1p-mediated retrieval from the golgi. (2004) Mol Biol Cell. 15, pp1417-1424. PMID 14699055.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08805pFET3-42Int.ApFET3-3xHA::URA3
RDB08806pFET3-43Int.ApFET3-GFP::URA3
RDB08807pFET3-52Int.ApFET3-3xHA::URA3::fet3(Δ5' region)Cut BamHI, then trafo
RDB08808pFET3-53Int.ApFET3-GFP::URA3::fet3(Δ5' region)Cut BamHI, then trafo
RDB08809pFET3-802NAInt.ApFET3(NheI-TMD-AflII)-3HA::URA3As RDB8805 except NheI & AflII at 560th & 584th codons, respectively
RDB08810pFET3-802L24Int.Apfet3[(TMD)560-584(24xL)]-3HA::URA3Cut NspV, then trafo
RDB08811pFET3-803NAInt.ApFET3(NheI-TMD-AflII)-GFP::URA3As RDB8806 except NheI & AflII at 560th & 584th codons, respectively.
RDB08812pFET3-803L24Int.Apfet3[(TMD)560-584(24xL)]-GFP::URA3Cut NspV, then trafo
RDB08813pFET3-42B-T565LInt.Apfet3(T565L)-3xHA ::URA3Cut NspV, then trafo
RDB08814pFET3-42B-S567LInt.Apfet3(S567L)-3xHA ::URA3Cut NspV, then trafo
RDB08815pFET3-42B-T577LInt.Apfet3(T577L)-3xHA ::URA3Cut NspV, then trafo
RDB08816pFET3-42B-Y581LInt.Apfet3(Y581L)-3xHA ::URA3Cut NspV, then trafo
RDB08817pFET3-43B-T565LInt.Apfet3(T565L)-3xHA ::URA3Cut NspV, then trafo
RDB08818pFET3-43B-S567LInt.Apfet3(S567L)-3xHA ::URA3Cut NspV, then trafo
RDB08819pFET3-43B-T577LInt.Apfet3(T577L)-GFP::URA3Cut NspV, then trafo
RDB08820pFET3-43B-Y581LInt.Apfet3(Y581L)-GFP::URA3Cut NspV, then trafo
RDB08821pTU1-FET3-3HACENURA3ApPTDH3-FET3-3xHA
RDB08822pTU1-FET3(L24)-3HACENURA3ApPTDH3-fet3[(TMD)560-584(24xL)]-3xHA::URA3
RDB08823pTU1-FET3(S567L)-3HACENURA3ApPTDH3-fet3(S567L)-3xHA

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Reconstitution of coat protein complex II (COPII) vesicle formation from cargo-reconstituted proteoliposomes reveals the potential role of GTP hydrolysis by Sar1p in protein sorting. (2004) J Biol Chem. 279, pp1330-1335. PMID 14627716.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08704pKSE131bacterialApStrep2-2xmyc-Emp47expression vector (tet promoter)
RDB08705pKSE132bacterialApStrep2-3xHA-Emp46expression vector (tet promoter)
RDB08706pKSE133bacterialApStrep2-myc-Emp47Δ(282-333)expression vector (tet promoter)
RDB08707pKSE136bacterialApMBP-Ufe1expression vector (tac promoter)

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Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. (2003) Mol Biol Cell. 14, pp3605-3616. PMID 12972550.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08580p[SEC12-MFα1] (S12M)TRP1ApSEC12-MFα1
RDB08581pRS316_MS71HCENURA3ApPSEC71-MFα1-SEC71-HA
RDB08582pYO326_MS71HURA3ApPSEC71-MFα1-SEC71-HA
RDB08583pRS316_MS63FCENURA3ApPSEC63-MFα1-SEC63-myc
RDB08584pYO326_MS63FURA3ApPSEC63-MFα1-SEC63-myc
RDB08585pSKY5wRER1-0CENTRP1ApPTDH3-EGFP-RER1-TCMK1
RDB08586pSKY5wRER1Y68LCENTRP1ApPTDH3-EGFP-rer1(Y68L)-TCMK1Rer1(Y68L)
RDB08587pSKY5wRER1N76LCENTRP1ApPTDH3-EGFP-rer1(N76L)-TCMK1Rer1(N76L)
RDB08588pSKY5wRER1Q77LCENTRP1ApPTDH3-EGFP-rer1(Q77L)-TCMK1Rer1(Q77L)
RDB08589pSKY5wRER1T83LCENTRP1ApPTDH3-EGFP-rer1(T83L)-TCMK1Rer1(T83L)
RDB08590pSKY5wRER1T128LCENTRP1ApPTDH3-EGFP-rer1(T128L)-TCMK1Rer1(T128L)
RDB08591pSKY5wRER1S131LCENTRP1ApPTDH3-EGFP-rer1(S131L)-TCMK1Rer1(S131L)
RDB08592pSKY5wRER1S135LCENTRP1ApPTDH3-EGFP-rer1(S135L)-TCMK1Rer1(S135L)
RDB08593pSKY5wRER1S138LCENTRP1ApPTDH3-EGFP-rer1(S138L)-TCMK1Rer1(S138L)
RDB08594pSKY5wRER1Y152LCENTRP1ApPTDH3-EGFP-rer1(Y152L)-TCMK1Rer1(Y152L)
RDB08595pSKY5wRER1T160LCENTRP1ApPTDH3-EGFP-rer1(T160L)-TCMK1Rer1(T160L)
RDB08598pQR316-SEC71-GFPCENURA3ApSEC71-GFP
RDB08599pQR326-SEC71-GFPURA3ApSEC71-GFP
RDB08600pRS306-SEC71TMD-GFP/LSInt.Apsec71T(LS)-GFP::URA3Sec71 (trans-membrane domain, L40S)
RDB08601pRS306-SEC71TMD-GFP/SLInt.Apsec71T(SL)-GFP::URA3Sec71 (trans-membrane domain, S43L)
RDB08602pRS306-SEC71TMD-GFP/YLInt.Apsec71T(YL)-GFP::URA3Sec71 (trans-membrane domain, Y36L)
RDB08603pRS306-SEC71TMD-GFP/1LInt.Apsec71T(1L)-GFP::URA3Sec71 (trans-membrane domain, 1xL insert at 41)
RDB08604pRS306-SEC71TMD-GFP/2LInt.Apsec71T(2L)-GFP::URA3Sec71 (trans-membrane domain, 2xL insert at 41)
RDB08605pRS306-SEC71TMD-GFP/3LInt.Apsec71T(3L)-GFP::URA3Sec71 (trans-membrane domain, 3xL insert at 41)
RDB08606pRS306-SEC71TMD-GFP/4LInt.Apsec71T(4L)-GFP::URA3Sec71 (trans-membrane domain, 4xL insert at 41)
RDB08607pRS306-SEC71TMD-GFP/2LΔLVInt.Apsec71T(2LΔLV)-GFP::URA3Sec71 (trans-membrane domain, L44Δ V45Δ 2xL insert at 41)
RDB08608pQR316-SEC71TMD-GFP/LSCENURA3Apsec71T(LS)-GFPSec71 (trans-membrane domain, L40S)
RDB08609pQR316-SEC71TMD-GFP/SLCENURA3Apsec71T(SL)-GFPSec71 (trans-membrane domain, S43L)
RDB08610pQR316-SEC71TMD-GFP/1LCENURA3Apsec71T(1L)-GFPSec71 (trans-membrane domain, 1xL insert at 41)
RDB08611pQR316-SEC71TMD-GFP/2LCENURA3Apsec71T(2L)-GFPSec71 (trans-membrane domain, 2xL insert at 41)
RDB08612pQR316-SEC71TMD-GFP/3LCENURA3Apsec71T(3L)-GFPSec71 (trans-membrane domain, 3xL insert at 41)
RDB08613pQR316-SEC71TMD-GFP/4LCENURA3Apsec71T(4L)-GFPSec71 (trans-membrane domain, 4xL insert at 41)
RDB08614pQR316-SEC71TMD-GFP/2LΔLVCENURA3Apsec71T(2LΔLV)-GFPSec71 (trans-membrane domain, L44Δ V45Δ 2xL insert at 41)
RDB08628pRS316-MWWWKCENURA3ApPSEC63-MFα1-3xHA-wbp1Wbp1 (C terminal 154 aa)
RDB08629pRS316-MWWWSCENURA3ApPSEC63-MFα1-3xHA-wbp1(SSTN)Wbp1 [C terminal 154aa, KKTN(C terminus)SSTN]
RDB08630pRS316-M71WWSCENURA3ApPSEC63-MFα1-3xHA-sec71L-wbp1T-wbp1C(SSTN)Sec71(luminal)-Wbp1(TM)-Wbp1[cytosolic, KKTN(C terminus)SSTN]
RDB08631pRS316-MW71WSCENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T-wbp1C(SSTN)Wbp1(luminal)-Sec71(TM)-Wbp1[cytosolic, KKTN(C terminus)SSTN]
RDB08632pRS316-MWW71CENURA3ApPSEC63-MFα1-3xHA-wbp1L-wbp1T-sec71CWbp1(luminal)-Wbp1(TM)-Sec71(cytosolic)
RDB08633pRS316-M71W71CENURA3ApPSEC63-MFα1-3xHA-sec71L-wbp1T-sec71CSec71(luminal)-Wbp1(TM)-Sec71(cytosolic)
RDB08634pRS316-MW71WS/LSCENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T(LS)-wbp1C(SSTN)Sec71 (trans-membrane domain, L40S)
RDB08635pRS316-MW71WS/YLCENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T(YL)-wbp1C(SSTN)Sec71 (trans-membrane domain, Y36L)
RDB08636pRS316-MW71WS/SLCENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T(SL)-wbp1C(SSTN)Sec71 (trans-membrane domain, S43L)
RDB08637pRS316-MW71WS/ΔLCENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T(ΔL)-wbp1C(SSTN)Sec71 (trans-membrane domain, L40Δ)
RDB08638pRS316-MW71WS/1LCENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T(1L)-wbp1C(SSTN)Sec71 (trans-membrane domain, 1xL insert at 41)
RDB08639pRS316-MW71WS/2LCENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T(2L)-wbp1C(SSTN)Sec71 (trans-membrane domain, 2xL insert at 41)
RDB08640pRS316-MW71WS/3LCENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T(3L)-wbp1C(SSTN)Sec71 (trans-membrane domain, 3xL insert at 41)
RDB08641pRS316-MW71WS/4LCENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T(4L)-wbp1C(SSTN)Sec71 (trans-membrane domain, 4xL insert at 41)
RDB08642pRS316-MW71WS/2LΔLVCENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T(2LΔLV)-wbp1C(SSTN)Sec71 (trans-membrane domain, L44Δ V45Δ 2xL insert at 41)
RDB08643pRS316-MW71WS/YSCENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T(YS)-wbp1C(SSTN)Sec71 (trans-membrane domain, Y36S)
RDB08644pRS316-MW71WS/YQCENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T(YQ)-wbp1C(SSTN)Sec71 (trans-membrane domain, Y36Q)
RDB08645pRS316-MW71WS/YACENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T(YA)-wbp1C(SSTN)Sec71 (trans-membrane domain, Y36A)
RDB08646pRS316-MW71WS/SYCENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T(SY)-wbp1C(SSTN)Sec71 (trans-membrane domain, S43Y)
RDB08647pRS316-MW71WS/SQCENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T(SQ)-wbp1C(SSTN)Sec71 (trans-membrane domain, S43Q)
RDB08648pRS316-MW71WS/SACENURA3ApPSEC63-MFα1-3xHA-wbp1L-sec71T(SA)-wbp1C(SSTN)Sec71 (trans-membrane domain, S43A)
RDB08650pRS316-mRFP-SEC12CENURA3ApmRFP-SEC12
RDB08651pRS316-GFP-SEC12CENURA3ApGFP-SEC12
RDB08652pRS306-SEC71TMD-GFPInt.ApURA3::sec71T-GFP::URA3Sec71(trans-membrane domain), localized to the ER at steady state in wild type cells.
RDB08653pQR316-SEC71TMD-GFPCENURA3Apsec71T-GFPSec71(trans-membrane domain) localized to the ER at steady state in wild type cells.
RDB08656pRS314-SEC71-mRFPCENTRP1ApSEC71-mRFP
RDB08657pSKY54-SEC12CENURA3ApGFP-SEC12

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Oligomerization of a cargo receptor directs protein sorting into COPII-coated transport vesicles. (2003) Mol Biol Cell. 14, pp3055-3063. PMID 12857885.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08683pKSY155CENTRP1ApGFP-myc-emp47(Δ383)Emp47Δ(383-C terminus)
RDB08684pKSY160CENTRP1ApGFP-myc-emp47(Δ333)Emp47Δ(333-C terminus)
RDB08685pKSY166CENTRP1ApGFP-myc-emp47(Δ281)Emp47Δ(281-C terminus)
RDB08686pKSY169TRP1Apmyc-EMP47
RDB08687pKSY177CENTRP1Apmyc-emp47(Δ281-333)Emp47Δ(281-333)
RDB08688pKSY187CENURA3ApHA-emp46(Δ279-321)Emp46Δ(279-321)

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Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. (2003) J Cell Biol. 161, pp1117-1131. PMID 12810702.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08559pKU1bacterialApTAT2cloning vector
RDB08560pKU41Int.Aptat2Δ::ADE2Cut EcoRI & SphI, then trafo
RDB08561pKU46CENURA3ApTAT2-3xHA
RDB08562pKU51Int.ApTAT2-3xHA::URA3Cut PmaCI, then trafo
RDB08563pKU60Int.Appep4Δ::HIS3Cut EcoRI & SalI, then trafo
RDB08564pKU62Int.Apvps1Δ::HIS3Cut SphI, then trafo
RDB08565pKU65Int.Apvps27Δ::HIS3Cut EcoRI & SalI, then trafo
RDB08566pKU68URA3ApTAT2
RDB08567pKU76CENURA3ApTAT2-GFPlocalized to the vacuole and perivacuolar late endosomes in the high tryptophan medium, and targeted to the plasma membrane in the tryptophan-free medium.
RDB08568pKU82Int.Aptat2Δ::hisG-URA3-hisGCut EcoRI & SphI, then trafo
RDB08569pKU84CENURA3ApGFP-PEP12resided mostly on the vacuolar-limiting membrane in wild-type cells.
RDB08570pKU105CENURA3ApPCUP1-ub-TCYC1ub: synthetic yeast ubiquitin
RDB08571pKU106CENURA3ApPCUP1-myc-ub-TCYC1ub: synthetic yeast ubiquitin
RDB08572pKU108Int.Apdoa4Δ::HIS3Cut SacI & SphI, then trafo
RDB08573pKU121Int.Apbul1Δ::HIS3Cut EcoRI & SalI, then trafo
RDB08574pKU131Int.Aperg13Δ::HIS3Cut EcoRI & SphI, then trafo
RDB08575pKU158CENURA3Aptat23KRTat2(K10R K17R K20R)

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Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae. (2002) Mol Biol Cell. 13, pp2518-2532. PMID 12134087.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08665pKSY103CENURA3ApEMP46
RDB08666pKSY104URA3ApEMP46
RDB08667pKSY105CENTRP1Ap2xmyc-EMP47
RDB08668pKSY113CENURA3Ap3xHA-EMP46
RDB08669pKSY114URA3Ap3xHA-EMP46
RDB08670pKSY115CENURA3Ap3xHA-emp46(ΔKVKLL)Emp46(ΔC-terminal KVKLL)
RDB08671pKSY116CENURA3Ap3xHA-emp46(RVRLL)Emp46[KVKLL(C-terminus)RVRLL]
RDB08672pKSY117CENURA3Ap3xHA-emp46(AYMF)Emp46[VKLL(C-terminus)AYMF]
RDB08673pKSY118CENURA3Ap3xHA-emp46(YYMA)Emp46[VKLL(C-terminus)YYMA]
RDB08674pKSY121CENURA3Ap3xHA-emp46(AYMA)Emp46[VKLL(C-terminus)AYMA]
RDB08675pKSY122CENURA3Ap3xHA-emp46(KVKAA)Emp46[KVKLL(C-terminus)KVKAA]
RDB08676pKSY123CENURA3Ap3xHA-emp46(SVSLL)Emp46[KVKLL(C-terminus)SVSLL]
RDB08677pKSY126CENURA3ApGFP-3xHA-EMP46
RDB08678pKSY131CENURA3ApGFP-3xHA-emp46(SVSLL)Emp46[KVKLL(C-terminus)SVSLL]
RDB08679pKSY132CENURA3ApGFP-3xHA-emp46(AYFM)Emp46[VKLL(C-terminus)AYFM]
RDB08680pKSY133CENURA3ApGFP-3xHA-emp46(YYMA)Emp46[VKLL(C-terminus)YYMA]
RDB08681pKSY134CENURA3ApGFP-3xHA-emp46(AYMA)Emp46[VKLL(C-terminus)AYMA]
RDB08682pKSY135CENURA3ApGFP-3xHA-emp46(KVKAA)Emp46[KVKLL(C-terminus)KVKAA]
RDB08693pKSE106bacterialApEmp46p (1-412)expression vector (T7 promoter, DE3 required)
RDB08694pKSE107Int.Apemp46Δ::HIS3
RDB08695pKSE108Int.Apemp47Δ::LEU2
RDB08696pKSE112bacterialApGST-Emp46Ctexpression vector (T7 promoter, DE3 required), Emp46(C-terminal 17AA)
RDB08697pKSE113bacterialApGST-Emp46Ct(AYMF)expression vector (T7 promoter, DE3 required), Emp46[C-terminal 17AA, VKLL(C-terminus)AYMF]
RDB08698pKSE114bacterialApGST-Emp46Ct(YYMA)expression vector (T7 promoter, DE3 required), Emp46[C-terminal 17AA, VKLL(C-terminus)YYMA]
RDB08699pKSE115bacterialApGST-Emp46Ct(AYMA)expression vector (T7 promoter, DE3 required), Emp46[C-terminal 17AA, VKLL(C-terminus)AYMA]
RDB08700pKSE116bacterialApGST-Emp46Ct(ΔKVKLL)expression vector (T7 promoter, DE3 required), Emp46(C-terminal 17AA, ΔC-terminal KVKLL)
RDB08701pKSE117bacterialApGST-Emp46Ct(KVKAA)expression vector (T7 promoter, DE3 required), Emp46[C-terminal 17AA, KVKLL(C-terminus)KVKAA]
RDB08702pKSE121bacterialApGST-Emp7Ctexpression vector (T7 promoter, DE3 required), Emp46(C-terminal 17AA)
RDB08703pKSE122bacterialApGST-Emp47Ct(STSLL)expression vector (T7 promoter, DE3 required), Emp46[C-terminal 17AA, KTKLL(C-terminus)STSLL]

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Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles in dolichol synthesis. (2001) Genes Cells. 6, pp495-506. PMID 11442630.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08824pR9-0bacterialApRER2cloning vector
RDB08825pΔrer2::HIS3Int.Aprer2Δ::HIS3Cut ScaI & EcoRV, then trafo
RDB08826pΔsrt1::ADE2Int.Apsrt1Δ::ADE2Cut XhoI & EcoRI, then trafo
RDB08827pΔsrt1::LEU2Int.Apsrt1Δ::LEU2Cut XhoI & PvuII, then trafo
RDB08828pSRT-2CENURA3ApSRT1
RDB08829pSRT-3URA3ApSRT1
RDB08830pSRTHA-2CENURA3ApSRT1-3xHA
RDB08831pSRTHA-3URA3ApSRT1-3xHA
RDB08832pSKY5-RER2CENURA3ApPTDH3-EGFP-RER2-TCMK1
RDB08833pSKY5-SRT1CENURA3ApPTDH3-EGFP-SRT1-TCMK1
RDB08834pEG6-4CENTRP1ApERG6-3xmyc

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Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer. (2001) J Cell Biol. 152, pp935-944. PMID 11238450.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08615pSKY5-RER1-0CENURA3ApPTDH3-EGFP-RER1-TCMK1punctate structures of the Golgi
RDB08616pSKY5-RER1-5CENURA3ApPTDH3-EGFP-rer1(-5)-TCMK1Rer1(ΔC-terminal 5aa)
RDB08617pSKY5-RER1-10CENURA3ApPTDH3-EGFP-rer1(-10)-TCMK1Rer1(ΔC-terminal 10aa)
RDB08618pSKY5-RER1-15CENURA3ApPTDH3-EGFP-rer1(-15)-TCMK1Rer1(ΔC-terminal 15aa)
RDB08619pSKY5-RER1-20CENURA3ApPTDH3-EGFP-rer1(-20)-TCMK1Rer1(ΔC-terminal 20aa)
RDB08620pSKY5-RER1-25CENURA3ApPTDH3-EGFP-rer1(-25)-TCMK1Rer1(ΔC-terminal 25aa)
RDB08621pSKY5-RER1 G179ACENURA3ApPTDH3-EGFP-rer1(G179A)-TCMK1Rer1(G179A)
RDB08622pSKY5-RER1-K180SCENURA3ApPTDH3-EGFP-rer1(K180S)-TCMK1Rer1(K180S)
RDB08623pSKY5-RER1-K181SCENURA3ApPTDH3-EGFP-rer1(K181S)-TCMK1Rer1(K181S)
RDB08624pSKY5-RER1-K182SCENURA3ApPTDH3-EGFP-rer1(K182S)-TCMK1Rer1(K182S)
RDB08625pSKY5-RER1-Y183ACENURA3ApPTDH3-EGFP-rer1(Y183A)-TCMK1Rer1(Y183A)
RDB08626pSKY5-RER1-Y173ACENURA3ApPTDH3-EGFP-rer1(Y173A)-TCMK1Rer1(Y173A)
RDB08627pSKY5-RER1-KKSSCENURA3ApPTDH3-EGFP-rer1(K180S K181S)-TCMK1Rer1(K180S K181S)
RDB08655pSKY5CENURA3ApPTDH3-EGFP-TCMK1
RDB08791p316-HA-STE2CENURA3ApPTDH3-HA-STE2-TCMK1
RDB08792p316-HA-ste2-rer1CENURA3ApPTDH3-HA-ste2-rer1-TCMK1Ste2(N-297)-Rer1(161-C)
RDB08793pGST-Wbp1bacterialApGST-Wbp1Ct (K421-N430)expression vector (tac prompoter)
RDB08794pGST-Wbp1-SSbacterialApGST-Wbp1Ct [K421-N430 (K427S K428S)]expression vector (tac prompoter)
RDB08795pGST-Rer1C28bacterialApGST-Rer1Ct (M161-N188)expression vector (tac prompoter)
RDB08796pGST-Rer1C28-KKSSbacterialApGST-Rer1Ct [M161-N188 (K180S K181S)]expression vector (tac prompoter)
RDB08797pGST-Rer1C28-Y173AbacterialApGST-Rer1Ct [M161-N188 (Y173A)]expression vector (tac prompoter)
RDB08798pGST-Rer1C28-Y183AbacterialApGST-Rer1Ct [M161-N188 (Y183A)]expression vector (tac prompoter)
RDB08799Pgap-Dap2URA3ApPTDH3-DAP2
RDB08800Pgap-DSDURA3ApPTDH3-dap2C-sec12T-dap2LDap2(cytosolic)-Sec12(TM)-Dap2(luminal)
RDB08801Pgap-DSD-NQ/LURA3ApPTDH3-dap2C-sec12T(N358L Q370L)-dap2LDap2(cytosolic)-Sec12(TM, N358L Q370L)-Dap2(luminal)
RDB08802Pgap-DSD-SY/LURA3ApPTDH3-dap2C-sec12T(S366L Y367L)-dap2LDap2(cytosolic)-Sec12(TM, N366L Q367L)-Dap2(luminal)
RDB08803Pgap-DSD-+2LURA3ApPTDH3-dap2C-sec12T(2xL ins 365)-dap2LDap2(cytosolic)-Sec12(TM, 2xL insert at 365)-Dap2(luminal)
RDB08804Pgap-DSD-L19URA3ApPTDH3-dap2C-(19xLeu)-dap2LDap2(cytosolic)-(19xLeu)-Dap2(luminal)

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Sed4p functions as a positive regulator of Sar1p probably through inhibition of the GTPase activation by Sec23p. (2000) Genes Cells. 5, pp1039-1048. PMID 11168590.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08664pYS28-E52CENTRP1ApSED4-3HA
RDB08766pYS6-7TRP1Apsar1(D32G)
RDB08767pYS6TRP1Apsar(E112K)
RDB08768pYS25URA3ApSED4
RDB08769pRS316-SEC16CENURA3ApSEC16
RDB08770pSKY2-2CENURA3ApPGAL1-Sec12 (1-354)-TCMK1

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Multiple roles of Arf1 GTPase in the yeast exocytic and endocytic pathways. (2001) Mol Biol Cell. 12, pp221-238. PMID 11160834.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08576YIpARF1Int.ApADE2::ARF1::ADE2Cut HpaI, then trafo
RDB08577p(arf1Δ::HIS3)Int.Aparf1Δ::HIS3Cut EcoRI & SphI, then trafo
RDB08578p(arf2Δ::HIS3)Int.Aparf2Δ::HIS3Cut PvuII, then trafo
RDB08579pNY14-A1SCENTRP1ApARF1
RDB08736pNY16-A1CENURA3ApEcoRI-ARF1
RDB08737pNY605CENTRP1Aparf1-11Arf1(K38I E132D L173S)
RDB08738pNY607NBCENTRP1Aparf1-12 Arf1(K38I)
RDB08739pNY801CENTRP1Aparf1-13 Arf1(L173S)
RDB08740pNY1004CENTRP1Aparf1-14 Arf1(F51I)
RDB08741pNY606NBCENTRP1Aparf1-15 Arf1(L25P)
RDB08743pNY902NBCENTRP1Aparf1-17Arf1(E41V)
RDB08744pNY1005CBCENTRP1Aparf1-18Arf1(H80P)
RDB08745p(605-ADE2)Int.ApADE2::arf1-11::ADE2Arf1(K38I E132D L173S)
RDB08746p(607NB-ADE2)Int.ApADE2::arf1-12::ADE2Arf1(K38I)
RDB08747p(801-ADE)Int.ApADE2::arf1-13::ADE2Arf1(L173S)
RDB08748p(606NB-ADE2)Int.ApADE2::arf1-14::ADE2Arf1(F51I)
RDB08749p(902-ADE2)Int.ApADE2::arf1-15::ADE2Arf1(L25P)
RDB08750p(902NB-ADE2)Int.ApADE2::arf1-17::ADE2Arf1(E41V)
RDB08751p(1005CB-ADE2)Int.ApADE2::arf1-18::ADE2Arf1(H80P)
RDB08931pNY902CENTRP1Aparf1-16 Arf1(E41V D129E)

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Introduction of Kex2 cleavage sites in fusion proteins for monitoring localization and transport in yeast secretory pathway. (2000) Methods Enzymol. 327, pp107-118. PMID 11044978.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08864pBAR1ΔHInt.Apbar1Δ::HIS3Cut SalI & XbaI, then trafo
RDB08865palpha1ΔA1Int.Apmfα1Δ::ADE2Cut EcoRI, then trafo
RDB08866palpha2ΔTInt.Apmfα2Δ::TRP1Cut SalI & BglII, then trafo

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Overexpression of PRA2, a Rab/Ypt-family small GTPase from Pea Pisum sativum, aggravates the growth defect of yeast ypt mutants. (2000) Cell Struct Funct. 25, pp11-20. PMID 10791890.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08752pYO773CENURA3ApPGAL1-(mcs)-TCMK1mcs: EcoRI BglII HindIII HpaI
RDB08753pNMV3CENHIS3ApPGAL1-(mcs)-TCMK1mcs: HpaI EcoRI BglII
RDB08754pNMV4CENTRP1ApPGAL1-(mcs)-TCMK1mcs: HpaI EcoRI BglII in mcs
RDB08755pGPR3CENURA3ApPGAL1-PRA2-TCMK1Pra2, origin: Pisum sativum
RDB08756pGPR3CCENURA3ApPGAL1-PRA2?C-TCMK1
RDB08757pGPR334CENURA3ApPGAL1-PRA2(T34N)-TCMK1Pra2(T34N), origin: Pisum sativum
RDB08758pGPR352CENURA3ApPGAL1-PRA2(T52A)-TCMK1Pra2(T52A), origin: Pisum sativum
RDB08759pGPR379CENURA3ApPGAL1-PRA2(Q79L)-TCMK1Pra2(Q79L), origin: Pisum sativum
RDB08760pGPR3133CENURA3ApPGAL1-PRA2(N133I)-TCMK1Pra2(N133I), origin: Pisum sativum
RDB08761pGGR3DCENURA3ApPGAL1-GDI1-TCMK1 PGAL1-PRA2-TCMK1Pra2, origin: Pisum sativum
RDB08762pGPR4CENHIS3ApPGAL1-PRA2-TCMK1Pra2, origin: Pisum sativum
RDB08763pGPR5CENTRP1ApPGAL1-PRA2-TCMK1Pra2, origin: Pisum sativum
RDB08764pNMY191CENURA3ApPGAL1-GDI1-TCMK1
RDB08765pDSS41CENURA3ApPGAL1-DSS4-TCMK1

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Evidence for recycling of cytochrome P450 sterol 14-demethylase from the cis-Golgi compartment to the endoplasmic reticulum (ER) upon saturation of the ER-retention mechanism. (2000) J Biochem. 127, pp747-754. PMID 10788782.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08788pYO326-chimeraNURA3ApNgs-ERG11-3xHANgs: potentiated N-linked glycosylation site [Erg11(L283MKNSTYKDGVKMTDQEIANLLIGVL312)]
RDB08789pRS316-chimeraNCENURA3ApNgs-ERG11-3xHANgs: potentiated N-linked glycosylation site [Erg11(L283MKNSTYKDGVKMTDQEIANLLIGVL312)]
RDB08790pYO326-ERG11/CYP51URA3ApERG11

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The inactive form of a yeast casein kinase I suppresses the secretory defect of the sec12 mutant. Implication of negative regulation by the Hrr25 kinase in the vesicle budding from the endoplasmic reticulum. (1999) J Biol Chem. 274, pp3804-3810. PMID 9920934.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08778pAM5-2TRP1Ap3xHA-HRR25
RDB08779pAM6-2TRP1Ap3xHA-hrr25-2as rst2-1, Hrr25(T176I)
RDB08780pAM4-1URA3ApPGAL1-3xHA-HRR25-TCYC1
RDB08781pAM8-2URA3ApPGAL1-3xHA-hrr25(K38A)-TCYC1
RDB08782pAM2-326URA3ApHRR25
RDB08783pAM1-5TRP1Aphrr25-2as rst2-1, Hrr25(T176I)
RDB08784pAM1-2CENTRP1Aphrr25-2as rst2-1, Hrr25(T176I)
RDB08785pAM8-1 (K38A)TRP1Aphrr25(K38A)
RDB08786pAM13-2CENURA3Apsec12-4Sec12(P73L)
RDB08787pAM13-3URA3Apsec12-4Sec12(P73L)

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Identification of SEC12, SED4, truncated SEC16, and EKS1/HRD3 as multicopy suppressors of ts mutants of Sar1 GTPase. (1999) J Biochem. 125, pp130-137. PMID 9880808.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08771pYO12LEU2ApSEC12
RDB08772pYOSARLEU2ApSAR1
RDB08773pANY4-2LEU2ApSED4
RDB08774pYS3URA3ApHRD3
RDB08775pYO112KS-1LEU2ApHRD3
RDB08776pYS1-6bacterialApHRD3cloning vector
RDB08777pYS2-2BInt.Aphrd3Δ::TRP1Cut KpnI & SacI, then trafo

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The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis. (1999) Mol Cell Biol. 19, pp471-483. PMID 9858571.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08835pY1URA3ApRER2
RDB08836p(rer2::LEU2)Int.Aprer2Δ::LEU2Cut HindIII, then trafo
RDB08837p(srt1::TRP1)Int.Apsrt1Δ::TRP1Cut XhoI & PvuII, then trafo
RDB08838pR7CENURA3ApRER2
RDB08839pR7HACENURA3Ap3xHA-RER2
RDB08840pR7HA-4URA3Ap3xHA-RER2
RDB08841pR7HA-2CENTRP1Ap3xHA-RER2

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Activities of mutant Sar1 proteins in guanine nucleotide binding, GTP hydrolysis, and cell-free transport from the endoplasmic reticulum to the Golgi apparatus. (1998) J Biochem. 124, pp816-823. PMID 9756629.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08876pMYE3-1bacterialApSar1expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08877pMYE3-2bacterialApSar1(K36M)expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08878pMYE3-3bacterialApSar1(D73V)expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08879pMYE3-4bacterialApSar1(H77L)expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08880pMYE3-5bacterialApSar1(N132I)expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08881pMYE3-6bacterialApSar1(C171S)expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08882pMYE3-7bacterialApSar1(D32G)expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08883pMYE3-8bacterialApSar1(T54A)expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08884pMYE3-9bacterialApSar1(E112K)expression vector (T7 promoter, DE3 required), cDNA derivative

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Characterization of yeast sar1 temperature-sensitive mutants, which are defective in protein transport from the endoplasmic reticulum. (1996) J Biochem. 120, pp452-458. PMID 8889833.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08914pTY3CENLEU2ApNcoI-SAR1genomic derivative (intron at 10th codon)
RDB08915pTY5CENLEU2ApPGAL1-SAR1genomic derivative (intron at 10th codon)
RDB08917pMYT3-7CENLEU2ApNcoI-sar1(D32G) cDNA derivative

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Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain. (1996) J Cell Biol. 134, pp279-93. PMID 8707815.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08842pMS2-3 (pSSS-1)bacterialApSEC12(D470E)-MFα1just for construction of RDB08844-RDB08846
RDB08843pDDD-2 (pSD1-2, pMD2-6)URA3ApDAP2-MFα1
RDB08844pDSS-2 (pSD4-2)URA3Apdap2L-sec12T-sec12C-MFα1Dap2(luminal)-Sec12(TM)-Sec12(cytosolic)
RDB08845pDDS-2 (pSD3-2)URA3Apdap2L-dap2T-sec12C-MFα1Dap2(luminal)-Dap2(TM)-Sec12(cytosolic)
RDB08846pSDS-2 (pSD2-2)URA3Apsec12L-dap2T-sec12C-MFα1Sec12(luminal)-Dap2(TM)-Sec12(cytosolic)
RDB08847pSSD-2 (pSD7-2)URA3Apsec12L-sec12T-dap2C-MFα1Sec12(luminal)-Sec12(TM)-Dap2(cytosolic)
RDB08848pDSD-2 (pSD5-2)URA3Apdap2L-sec12T-dap2C-MFα1Dap2(luminal)-Sec12(TM)-Dap2(cytosolic)
RDB08849pSDD-2 (pSD6-2)URA3Apsec12L-dap2T-dap2C-MFα1Sec12(luminal)-Dap2(TM)-Sec12(cytosolic)
RDB08850pDSDm F-AURA3Apdap2L-sec12T(F355A F356A F359A)-dap2C-MFα1Dap2(luminal)-Sec12(TM, F355A F356A F359A)-Dap2(cytosolic)
RDB08851pDSDm SY-AURA3Apdap2L-sec12T(S366A Y367A S372A)-dap2C-MFα1Dap2(luminal)-Sec12(TM, S366A Y367A S372A)-Dap2(cytosolic)
RDB08852pDSDm N-AURA3Apdap2L-sec12T(N358A)-dap2C-MFα1Dap2(luminal)-Sec12(TM, N358A)-Dap2(cytosolic)
RDB08853pDSDm Q-AURA3Apdap2L-sec12T(Q370A)-dap2C-MFα1Dap2(luminal)-Sec12(TM, Q370A)-Dap2(cytosolic)
RDB08854pDSDm NQ-AURA3Apdap2L-sec12T(N358A Q370A)-dap2C-MFα1Dap2(luminal)-Sec12(TM, N358A Q370A)-Dap2(cytosolic)
RDB08855pDSDm A10URA3Apdap2L-sec12T(F355A F356A T357A N358A F369A S366A Y367A Q370A F371A S372A)-dap2C-MFα1Dap2(luminal)-Sec12(TM, F356A F357A T358A N359A F360A S366A Y367A Q370A F371A S372A)-Dap2(cytosolic)
RDB08856pDSDm N-LURA3Apdap2L-sec12T(N358L)-dap2C-MFα1Dap2(luminal)-Sec12(TM, N358L)-Dap2(cytosolic)
RDB08857pDSDm Q-LURA3Apdap2L-sec12T(Q370L)-dap2C-MFα1Dap2(luminal)-Sec12(TM, Q370L)-Dap2(cytosolic)
RDB08858pDSDm NQ-LURA3Apdap2L-sec12T(N358L Q370L)-dap2C-MFα1Dap2(luminal)-Sec12(TM, N358L Q370L)-Dap2(cytosolic)
RDB08859pDSDm-L7URA3Apdap2L-sec12T(T357L N358L S366L Y367L Q370L S372L)-dap2C-MFα1Dap2(luminal)-Sec12(TM, T357L N358L S366L Y367L Q370L S372L)-Dap2(cytosolic)
RDB08860p(LeuX19)URA3Apdap2L-(19xLeu)-dap2C-MFα1Dap2(luminal)-(19xLeu)-Dap2(cytosolic)
RDB08861pD4Dm-2URA3Apdap2L-sed4T-dap2C-MFα1Dap2(luminal)-Sed4(TM)-Dap2(cytosolic)
RDB08862pD20Dm-2URA3Apdap2L-sec20T-dap2C-MFα1Dap2(luminal)-Sec20(TM)-Dap2(cytosolic)
RDB08863p4DDm-2URA3Apsed4L-dap2T-dap2C-MFα1Sed4(luminal)-Dap2(TM)-Dap2(cytosolic)

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Membrane protein retrieval from the Golgi apparatus to the endoplasmic reticulum (ER): characterization of the RER1 gene product as a component involved in ER localization of Sec12p. (1995) Mol Biol Cell. 6, pp1459-1477. PMID 8589449.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08596pYO324/RER1-3HATRP1ApRER1-3HA
RDB08597pYO324/RER1-3HA Y152LTRP1Aprer1(Y152L)-3HARer1(Y152L)
RDB08649pSK/rer1::LEU2Int.Aprer1Δ::LEU2
RDB08654pRS314/RERI-3HACENTRP1ApRER1-3HA

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Identification of a gene required for membrane protein retention in the early secretory pathway. (1993) Proc Natl Acad Sci U S A. 90, pp8179-8183. PMID 8367481.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08870pSHF2-1bacterialApSEC12cloning vector
RDB08871pSHY6-3URA3ApSEC12
RDB08872pSHF9-4URA3ApSEC12-MFα1Δ(1-10)MFα1(signal peptide truncated)
RDB08873pSHF9-4ΔSalURA3Apsec12(1-275)-MFα1Δ(1-10)MFα1(signal peptide truncated)
RDB08874pSHF11-1URA3ApSEC12(D470E)-SUC2Δ(1-21)Suc2(signal peptide truncated)

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Inhibition of endoplasmic reticulum (ER)-to-Golgi transport induces relocalization of binding protein (BiP) within the ER to form the BiP bodies. (1994) Mol Biol Cell. 5, pp1129-1143. PMID 7865879.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08867pSHY13-2URA3ApPGAL1-SEC12
RDB08868pSHY14-5URA3ApPGAL1-SED4
RDB08869pSHY15-1TRP1ApPGAL1-SEC23-TCMK1

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Mutational analysis of the Sar1 protein, a small GTPase which is essential for vesicular transport from the endoplasmic reticulum. (1994) J Biochem. 116, pp243-247. PMID 7822237.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08885pMYY3-1CENTRP1ApSAR1cDNA derivative
RDB08886pMYY3-2CENTRP1Apsar1(K36M)cDNA derivative
RDB08887pMYY3-3CENTRP1Apsar1(D73V)cDNA derivative
RDB08888pMYY3-4CENTRP1Apsar1(H77L)cDNA derivative
RDB08889pMYY3-5CENTRP1Apsar1(N132I)cDNA derivative
RDB08890pMYY3-6CENTRP1Apsar1(C171S)cDNA derivative
RDB08891pMYY3-7CENTRP1Apsar1(D32G)cDNA derivative
RDB08892pMYY3-8CENTRP1Apsar1(T54A)cDNA derivative
RDB08893pMYY3-9CENTRP1Apsar1(E112K)cDNA derivative
RDB08894pMYY4-1CENTRP1ApPGAL1-SAR1cDNA derivative
RDB08895pMYY4-2CENTRP1ApPGAL1-sar1(K36M)cDNA derivative
RDB08896pMYY4-3CENTRP1ApPGAL1-sar1(D73V)cDNA derivative
RDB08897pMYY4-4CENTRP1ApPGAL1-sar1(H77L)cDNA derivative
RDB08898pMYY4-5CENTRP1ApPGAL1-sar1(N132I)cDNA derivative
RDB08899pMYY4-6CENTRP1ApPGAL1-sar1(C171S)cDNA derivative
RDB08900pMYY4-7CENTRP1ApPGAL1-sar1(D32G)cDNA derivative
RDB08901pMYY4-8CENTRP1ApPGAL1-sar1(T54A)cDNA derivative
RDB08902pMYY4-9CENTRP1ApPGAL1-sar1(E112K)cDNA derivative

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A membrane glycoprotein, Sec12p, required for protein transport from the endoplasmic reticulum to the Golgi apparatus in yeast. (1988) J Cell Biol. 107, pp851-863. PMID 3047151.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08903pANY1-4CENURA3ApSEC12
RDB08904pANY1-8Int.Apsec12Δ(274-C)::URA3::sec12Δ(N-47)Cut KpnI, then trafo
RDB08905pANY1-9URA3ApSEC12
RDB08916pANF1bacterialAplacZ-Sec12(48-Cterm)expression vector (lac promoter), antigen for Sec12p antibody

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A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus. (1989) J Cell Biol. 109, pp2677-2691. PMID 2512296.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08906pANY2-7URA3ApSAR1gemomic (intron at 10th codon)
RDB08907pANY2-9CENURA3ApSAR1gemomic (intron at 10th codon)
RDB08908pANY2-17CENTRP1ApSAR1gemomic (intron at 10th codon)
RDB08909pANY2-18CENTRP1ApPGAL1-SAR1gemomic (intron at 10th codon)
RDB08910pANY2-19TRP1ApPGAL1-SAR1gemomic (intron at 10th codon)
RDB08911pANY3-11CENTRP1ApPGAL1-SAR1cDNA
RDB08912pANY3-14bacterialApSAR1cloning vector, cDNA

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The GTP-binding Sar1 protein is localized to the early compartment of the yeast secretory pathway. (1991) Biochim. Biophys. Acta. 1093, pp135-143. PMID 1907491.
Catalog No.plasmidbackborneSc markerEc markerinsertremarks
RDB08875pSHY1-1TRP1ApSAR1gemomic (intron at 10th codon)
RDB08913pANF31bacterialAplacZ-Sar1(42-190)expression vector (lac promoter), antigen for Sar1p antibody

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(2014.07.16 K.N., T.M.)

2019.01.11