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52/E04 - S. pombe Postgenome Database Clone

Available from Ordering information ordering information
Clone ID at the S. pombe Postgenome Database 52/E04
NCBI Gene ID 5802726
Gene Symbol spc19
Protein Name DASH complex subunit Spc19
Synonym SPCC1223.15c
RefSeq Protein
PomBase(1) link 01
KEGG(2) link 01
Depositor-supplied data(3) link 01
Note  

Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Entry vector

Catalog number SPW020900
Clone name SpENT52E04
Clone status Difference in sequence of reverse PCR primer region: AGAAAGCTGGGT(A:null)AATGACCTCGTTTTTGTTG. (see depositors data)

FFH Vector(5)

Catalog number SPW060900
Clone name SpFFH52E04
Clone status confirmation required

YFH Vector(6)

Catalog number SPW100900
Clone name SpYFH52E04
Clone status Difference in sequence of reverse PCR primer region: AGAAAGCTGGGT(A:null)AATGACCTCGTTTTTGTTG. (see depositors data)

Please note that they are GENOMIC clones.
Please visit plasmidData Sheet for the information of vectors and sequence primers.

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(5) FLAG2-His6-tag clone.

(6) YFP-FLAG-His6-tag clone.

(7) Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).


2016.09.01

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_160723.pl