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52/E04 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 52/E04
NCBI Gene ID 5802726
Gene Symbol spc19
Protein Name DASH complex subunit Spc19
Synonym SPCC1223.15c
RefSeq Protein
PomBase(1) SPCC1223.15c
KEGG(2) spo:SPCC1223.15c
Depositor-supplied data(3) 52E04
Note  

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW020900 SpENT52E04 Difference in sequence of reverse PCR primer region: AGAAAGCTGGGT(A:null)AATGACCTCGTTTTTGTTG. (see depositors data)

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW060900 SpFFH52E04 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW100900 SpYFH52E04 Difference in sequence of reverse PCR primer region: AGAAAGCTGGGT(A:null)AATGACCTCGTTTTTGTTG. (see depositors data)

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2023.11.07

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl