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50/F12 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 50/F12
NCBI Gene ID 2543227
Gene Symbol rvb1
Protein Name AAA family ATPase Rvb1
Synonym SPAPB8E5.09
RefSeq Protein NP_594783.1
PomBase(1) SPAPB8E5.09
KEGG(2) spo:SPAPB8E5.09
Depositor-supplied data(3) 50F12
Note  

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number SPW020132
Clone name SpENT50F12
Clone status confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number SPW060132
Clone name SpFFH50F12
Clone status confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number SPW100132
Clone name SpYFH50F12
Clone status available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH50F12a.seq SpYFH50F12b.seq   SpYFH50F12.pdf

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2018.10.13

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_171016.pl