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49/H06 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 49/H06
NCBI Gene ID 2539195
Gene Symbol bfr1
Protein Name brefeldin A efflux transporter Bfr1
Synonym SPCC18B5.01c|hba2,SPCPJ732.04c
RefSeq Protein NP_587932.2
PomBase(1) SPCC18B5.01c
KEGG(2) spo:SPCC18B5.01c
Depositor-supplied data(3) 49H06
Note 3462G:A / 3594T:C / 3857A:G(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW019774 SpENT49H06 confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW059774 SpFFH49H06 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW099774 SpYFH49H06 available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH49H06a.seq SpYFH49H06b.seq   SpYFH49H06.pdf
user_report - reference Wang, M., Engineering an ABC transporter for enhancing resistance to caffeine in Saccharomyces cerevisiae. J Agric Food Chem. 64 (42): 7973–7978 (2016). PMID 27696877.

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


pombe_clone_primer_ver2_2023Apr26.csv -