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49/E05 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 49/E05
NCBI Gene ID 2542014
Gene Symbol mfh2
Protein Name ATP-dependent 3' to 5' DNA helicase (predicted)
Synonym SPAC20H4.04
RefSeq Protein
PomBase(1) SPAC20H4.04
KEGG(2) spo:SPAC20H4.04
Depositor-supplied data(3) 49E05
Note 297A:G / 953A:T / 1220T:C / 1588G:C(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number SPW019701
Clone name SpENT49E05
Clone status confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number SPW059701
Clone name SpFFH49E05
Clone status confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number SPW099701
Clone name SpYFH49E05
Clone status available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH49E05a.seq SpYFH49E05b.seq   SpYFH49E05.pdf

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2018.10.13

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_171016.pl