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48/H07 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 48/H07
NCBI Gene ID 2543610
Gene Symbol sec71
Protein Name Sec7 domain protein, ARF GEF (predicted)
Synonym SPAC4D7.01c|sec7a,SPAP8A3.15c
RefSeq Protein NP_594954.1
PomBase(1) SPAC4D7.01c
KEGG(2) spo:SPAC4D7.01c
Depositor-supplied data(3) 48H07
Note  

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW019375 SpENT48H07 available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpENT48H07a.seq SpENT48H07b.seq   SpENT48H07.pdf

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW059375 SpFFH48H07 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW099375 SpYFH48H07 available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH48H07a.seq SpYFH48H07b.seq   SpYFH48H07.pdf

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2023.11.07

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl