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47/F11 - S. pombe Postgenome Database Clone

Available from Ordering information ordering information
Clone ID at the S. pombe Postgenome Database 47/F11
NCBI Gene ID 2538760
Gene Symbol rad16
Protein Name DNA repair endonuclease XPF
Synonym SPCC970.01|rad10,rad20,swi9
RefSeq Protein NP_587855.1
PomBase(1) link 01
KEGG(2) link 01
Depositor-supplied data(3) link 01
Note  

Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Entry vector

Catalog number SPW018931
Clone name SpENT47F11
Clone status confirmation required

FFH Vector(5)

Catalog number SPW058931
Clone name SpFFH47F11
Clone status confirmation required

YFH Vector(6)

Catalog number SPW098931
Clone name SpYFH47F11
Clone status confirmation required
user report - reference Zhang, J.M., Fission yeast Pxd1 promotes proper DNA repair by activating Rad16XPF and inhibiting Dna2. PLoS Biol. 12 (9): e1001946 (2014). PMID 25203555.

Please note that they are GENOMIC clones.
Please visit plasmidData Sheet for the information of vectors and sequence primers.

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(5) FLAG2-His6-tag clone.

(6) YFP-FLAG-His6-tag clone.

(7) Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).


2017.01.30

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_160723.pl