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47/C03 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 47/C03
NCBI Gene ID 2542327
Gene Symbol upf1
Protein Name ATP-dependent RNA helicase Upf1
Synonym SPAC16C9.06c
RefSeq Protein NP_593080.1
PomBase(1) SPAC16C9.06c
KEGG(2) spo:SPAC16C9.06c
Depositor-supplied data(3) 47C03
Note  

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number SPW018851
Clone name SpENT47C03
Clone status confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number SPW058851
Clone name SpFFH47C03
Clone status available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpFFH47C03a.seq SpFFH47C03b.seq   SpFFH47C03.pdf

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number SPW098851
Clone name SpYFH47C03
Clone status available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH47C03a.seq SpYFH47C03b.seq   SpYFH47C03.pdf

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2018.10.13

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_171016.pl