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47/B10 - S. pombe Postgenome Database Clone

Available from Ordering information ordering information
Clone ID at the S. pombe Postgenome Database 47/B10
NCBI Gene ID 2542393
Gene Symbol cul1
Protein Name cullin 1
Synonym SPAC17G6.12|pcu1
RefSeq Protein NP_594259.1
PomBase(1) link 01
KEGG(2) link 01
Depositor-supplied data(3) link 01

Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Entry vector

Catalog number SPW018834
Clone name SpENT47B10
Clone status confirmation required

FFH Vector(5)

Catalog number SPW058834
Clone name SpFFH47B10
Clone status confirmation required
user report - reference Girdwood, D., Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe. FEBS Lett., 586 (10): 1522-1528 (2012). PMID 22673520.

YFH Vector(6)

Catalog number SPW098834
Clone name SpYFH47B10
Clone status confirmation required

Please note that they are GENOMIC clones.
Please visit plasmidData Sheet for the information of vectors and sequence primers.

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(5) FLAG2-His6-tag clone.

(6) YFP-FLAG-His6-tag clone.

(7) Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).


pombe_clone_primer_ver2_160708_xlsx.csv -