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46/B08 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 46/B08
NCBI Gene ID 2541442
Gene Symbol SPAC22A12.08c
Protein Name cardiolipin synthase/ hydrolase fusion protein (predicted)
Synonym
RefSeq Protein
PomBase(1) SPAC22A12.08c
KEGG(2) spo:SPAC22A12.08c
Depositor-supplied data(3) 46B08
Note  

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW018432 SpENT46B08 confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW058432 SpFFH46B08 available
Seq File
(by primer A)		 Seq File
(by primer B)		 Seq File
(by primer C)		 PDF File
SpFFH46B08a.seq SpFFH46B08b.seq   SpFFH46B08.pdf
user_report - reference Vircikova, V., Schizosaccharomyces pombe cardiolipin synthase is part of a mitochondrial fusion protein regulated by intron retention. Biochim Biophys Acta pii: S1388-1981 (18) 30146-X (2018). PMID 29958934.

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW098432 SpYFH46B08 confirmation required

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.

2023.11.07

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl