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45/H04 - S. pombe Postgenome Database Clone

Available from Ordering information ordering information
Clone ID at the S. pombe Postgenome Database 45/H04
NCBI Gene ID 2538866
Gene Symbol SPCC1620.12c
Protein Name GTPase activating protein (predicted)
Synonym
RefSeq Protein
PomBase(1) link 01
KEGG(2) link 01
Depositor-supplied data(3) link 01
Note  

Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Entry vector

Catalog number SPW018172
Clone name SpENT45H04
Clone status confirmation required

FFH Vector(5)

Catalog number SPW058172
Clone name SpFFH45H04
Clone status confirmation required

YFH Vector(6)

Catalog number SPW098172
Clone name SpYFH45H04
Clone status confirmation required
user report - reference Weston, L., Genome-wide screen for cell growth regulators in fission yeast. J Cell Sci. 2017 May 5. pii: jcs.200865. doi: 10.1242/jcs.200865. [Epub ahead of print] PMID 28476936.

Please note that they are GENOMIC clones.
Please visit plasmidData Sheet for the information of vectors and sequence primers.

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(5) FLAG2-His6-tag clone.

(6) YFP-FLAG-His6-tag clone.

(7) Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).


2017.06.20

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_160723.pl