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45/G09 - S. pombe Postgenome Database Clone

Available from Ordering information ordering information
Clone ID at the S. pombe Postgenome Database 45/G09
NCBI Gene ID 2539087
Gene Symbol chk1
Protein Name Chk1 protein kinase
Synonym SPCC1259.13|rad27
RefSeq Protein NP_588070.1
PomBase(1) link 01
KEGG(2) link 01
Depositor-supplied data(3) link 01
Note  

Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Entry vector

Catalog number SPW018153
Clone name SpENT45G09
Clone status confirmation required

FFH Vector(5)

Catalog number SPW058153
Clone name SpFFH45G09
Clone status confirmation required

YFH Vector(6)

Catalog number SPW098153
Clone name SpYFH45G09
Clone status confirmation required
user report - reference Qu, M., Phosphorylation-dependent interactions between Crb2 and Chk1 are essential for DNA damage checkpoint. PLoS Genet. 8 (7): e1002817 (2012). PMID 22792081.

Please note that they are GENOMIC clones.
Please visit plasmidData Sheet for the information of vectors and sequence primers.

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(5) FLAG2-His6-tag clone.

(6) YFP-FLAG-His6-tag clone.

(7) Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).


2017.01.30

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_160723.pl