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44/C02 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 44/C02
NCBI Gene ID 2543243
Gene Symbol mel1
Protein Name alpha-galactosidase, melibiase
Synonym SPAC869.07c
RefSeq Protein NP_595012.1
PomBase(1) SPAC869.07c
KEGG(2) spo:SPAC869.07c
Depositor-supplied data(3) 44C02
Note  

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number SPW017650
Clone name SpENT44C02
Clone status confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number SPW057650
Clone name SpFFH44C02
Clone status confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number SPW097650
Clone name SpYFH44C02
Clone status available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH44C02a.seq SpYFH44C02b.seq   SpYFH44C02.pdf

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2018.10.13

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_171016.pl