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41/B03 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 41/B03
NCBI Gene ID 2540719
Gene Symbol rad3
Protein Name ATR checkpoint kinase Rad3
Synonym SPBC216.05
RefSeq Protein NP_595357.1
PomBase(1) SPBC216.05
KEGG(2) spo:SPBC216.05
Depositor-supplied data(3) 41B03
Note 3526T:C / 4071C:T / 5235A:C / 5655T:C / 7082A:G(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW016427 SpENT41B03 available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpENT41B03a.seq SpENT41B03b.seq   SpENT41B03.pdf

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW056427 SpFFH41B03 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW096427 SpYFH41B03 confirmation required

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2023.11.07

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl