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41/B01 - S. pombe Postgenome Database Clone

Available from Ordering information ordering information
Clone ID at the S. pombe Postgenome Database 41/B01
NCBI Gene ID 2540292
Gene Symbol rpb1
Protein Name RNA polymerase II large subunit Rpb1
Synonym SPBC28F2.12
RefSeq Protein NP_595673.1
PomBase(1) link 01
KEGG(2) link 01
Depositor-supplied data(3) link 01
Note  

Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Entry vector

Catalog number SPW016425
Clone name SpENT41B01
Clone status confirmation required

FFH Vector(5)

Catalog number SPW056425
Clone name SpFFH41B01
Clone status confirmation required

YFH Vector(6)

Catalog number SPW096425
Clone name SpYFH41B01
Clone status confirmation required
user report - reference Weston, L., Genome-wide screen for cell growth regulators in fission yeast. J Cell Sci. 2017 May 5. pii: jcs.200865. doi: 10.1242/jcs.200865. [Epub ahead of print] PMID 28476936.

Please note that they are GENOMIC clones.
Please visit plasmidData Sheet for the information of vectors and sequence primers.

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(5) FLAG2-His6-tag clone.

(6) YFP-FLAG-His6-tag clone.

(7) Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).


2017.06.20

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_160723.pl